ADSORPTION BEHAVIOR OF FIBRONECTIN ON WELL-CHARACTERIZED SILICA SURFACES
ADSORPTION BEHAVIOR OF FIBRONECTIN ON WELL-CHARACTERIZED SILICA SURFACES
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DOI：
10.1016/0021-9797(82)90408-8
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发表时间：
1982-01-01
影响因子：
9.9
通讯作者：
BERGHEM, L
中科院分区：
文献类型：
--
作者：
JONSSON, U;IVARSSON, B;BERGHEM, L
The adsorption behavior of human plasma fibronectin at physiological pH and ionic strength was investigated by in situ ellipsometry on silica substrates of 2 different surface energies. The silica substrate was characterized by ellipsometry, galvanic potential, and critical surface tension measurements. Adsorption isotherms in the low concentration range for fibronectin were compared with human fibrinogen and found to be qualitatively similar with increased amounts of protein adsorbed at the plateau on a hydrophobic surface as compared to a hydrophilic one. Reversibility for fibronectin upon dilution was small on a hydrophobic surface while a partial desorption was found on a hydrophilic one. Interaction of antibodies with preadsorbed fibronectin suggests that fibronectin adsorbs in different conformations and/or arrangements on the 2 types of surfaces. The lectin Concanavalin A and its interaction with preadsorbed fibronectin was also investigated.
