ATP plays a role in neurite stimulation with activated mast cells
ATP plays a role in neurite stimulation with activated mast cells
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DOI：
10.1016/j.jneuroim.2007.09.013
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发表时间：
2007-12-01
影响因子：
3.3
通讯作者：
Nakamshi, Mamoru
中科院分区：
文献类型：
--
作者：
Suzuki, Ryo;Furuno, Tadahide;Nakamshi, Mamoru
此前，我们已表明神经与肥大细胞之间可发生双向信号交流，且P物质是激活肥大细胞的一种介质。在此，我们研究了激活与肥大细胞共培养的神经的介质。将抗原添加到颈上神经节（SCG）与大鼠嗜碱性粒细胞白血病细胞（RBLs）的共培养体系中，可引发RBLs内的钙离子响应，经过一段延迟期后，还会诱导SCG神经突内产生钙离子信号。磷酸吡哆醛 - 6 - 偶氮苯 - 2',4'-二磺酸（嘌呤能受体拮抗剂）或腺苷三磷酸双磷酸酶（ATP水解酶）可减弱神经突内的钙离子信号，这表明活化的肥大细胞释放的ATP是激活神经的重要介质之一。（c）2007爱思唯尔出版社。保留所有权利。
Previously, we showed that nerve-mast cell cross-talk can occur bidirectionally and that substance P is a mediator to activate mast cells. Here, we have studied the mediators to activate nerves cocultured with mast cells. Addition of antigen to the cocultures of superior cervical ganglia (SCG) and rat basophilic leukemia cells (RBLs) elicited Ca2+ response in RBLs and after a lag period induced Ca2+ signal in SCG neurites. Pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (purinergic receptor antagonist) or apyrase (ATP-hydrolyzing enzyme) reduced the Ca2+ signals in neurites, indicating that ATP released from activated rnast cells was one of important mediators to activate nerves. (c) 2007 Elsevier B.V. All rights reserved.