Citric acid pretreatment for suppressing adhesion of major egg allergens to a stainless steel surface
Citric acid pretreatment for suppressing adhesion of major egg allergens to a stainless steel surface
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DOI：
10.1016/j.foodcont.2013.02.002
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发表时间：
2013-08
期刊：
影响因子：
6
通讯作者：
T. Sakiyama;Kentaro Sato;Sanae Tsuda;H. Sugiyama;T. Hagiwara
中科院分区：
文献类型：
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作者：
T. Sakiyama;Kentaro Sato;Sanae Tsuda;H. Sugiyama;T. Hagiwara
蛋白质在固体表面的粘附会在食品制造中引起许多问题。特别是，少量粘附的过敏蛋白可能会造成交叉污染的风险。为了探索一种抑制鸡蛋主要过敏原在不锈钢表面粘附的策略，研究了柠檬酸预处理不锈钢表面在各种条件下对卵清蛋白和卵类粘蛋白粘附的影响。在用50 mM柠檬酸溶液处理、用水冲洗和干燥后，对不锈钢粉末进行粘附实验。在30 °C和pH 7.4下的卵清蛋白粘附的结果表明，柠檬酸比测试的任何单价或二价有机酸更有效地对抗卵清蛋白粘附。这支持了这样的想法，即粘附的抑制归因于附着在不锈钢表面的酸阴离子，并产生有效的负表面电荷。柠檬酸预处理在较高pH 9.0和80 °C的温度下也抑制卵清蛋白的粘附。卵类粘蛋白的粘附在30和80 °C下也被柠檬酸预处理抑制。这些结果表明，柠檬酸预处理对主要鸡蛋过敏原粘附到不锈钢表面是有效的。
Adhesion of proteins to solid surfaces can cause a number of problems in food manufacturing. In particular, a tiny amount of adherent allergenic proteins may pose a cross-contamination risk. To explore a strategy for suppressing adhesion of major egg allergens to stainless steel surfaces, the effect of citric acid pretreatment of stainless steel surfaces on the adhesion of ovalbumin and ovomucoid was studied under various conditions. Stainless steel powder was subjected to adhesion experiments after treatment with 50 mM citric acid solution, rinsing with water, and drying. Results from ovalbumin adhesion at 30 °C and pH 7.4 demonstrate that citric acid was more effective against ovalbumin adhesion than any univalent or divalent organic acid tested. This supports the idea that the suppression of adhesion is ascribed to the acid anions attaching to the stainless steel surface and yielding an effective negative surface charge. Citric acid pretreatment also suppressed the adhesion of ovalbumin at higher pH of 9.0 and temperature of 80 °C. The adhesion of ovomucoid was also suppressed at both 30 and 80 °C by citric acid pretreatment. These results suggest that the citric acid pretreatment is effective against the adhesion of major egg allergens to stainless steel surfaces.