An SNW/SKI-INTERACTING PROTEIN influences endoreduplication and cell growth in Arabidopsis
An SNW/SKI-INTERACTING PROTEIN influences endoreduplication and cell growth in Arabidopsis
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DOI：
10.1093/plphys/kiac415
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发表时间：
2022-09-05
期刊：
影响因子：
7.4
通讯作者：
Li, Yunhai
中科院分区：
文献类型：
--
作者：
Jiang, Shan;Meng, Bolun;Li, Yunhai
核内复制在细胞生长和分化过程中起着重要作用，但调控核内复制的机制尚不清楚。我们之前报道过由DA3编码的UBIQUITIN-SPECIFIC PROTEASE14 （UBP14）与ultraviolet INSENSITIVE4 （UVI4）相互作用，影响拟南芥（Arabidopsis thaliana）的内复制和细胞生长。与野生型相比，da3-1突变体具有更大的子叶和更高的倍性水平。在这里，我们鉴定了da3-1的抑制因子（suppressor of da3-1 3; SUD3），它编码SNW/ ski相互作用蛋白（SKIP）。生化研究表明，SUD3在体内和体外均与UBP14/DA3和UVI4发生物理相互作用。遗传分析支持SUD3与UBP14/DA3和UVI4通过共同途径控制内复制。我们的研究结果揭示了SKIP/SUD3与UBP14/DA3和UVI4联合调节内复制的重要遗传和分子机制。SNW/ ski - interaction PROTEIN与泛素特异性蛋白酶14和紫外- b INSENSITIVE4相互作用，影响拟南芥内复制和细胞生长。
Endoreduplication plays an important role in cell growth and differentiation, but the mechanisms regulating endoreduplication are still elusive. We have previously reported that UBIQUITIN-SPECIFIC PROTEASE14 (UBP14) encoded by DA3 interacts with ULTRAVIOLETB INSENSITIVE4 (UVI4) to influence endoreduplication and cell growth in Arabidopsis (Arabidopsis thaliana). The da3-1 mutant possesses larger cotyledons and flowers with higher ploidy levels than the wild-type. Here, we identify the suppressor of da3-1 (SUPPRESSOR OF da3-1 3; SUD3), which encodes SNW/SKI-INTERACTING PROTEIN (SKIP). Biochemical studies demonstrate that SUD3 physically interacts with UBP14/DA3 and UVI4 in vivo and in vitro. Genetic analyses support that SUD3 acts in a common pathway with UBP14/DA3 and UVI4 to control endoreduplication. Our findings reveal an important genetic and molecular mechanism by which SKIP/SUD3 associates with UBP14/DA3 and UVI4 to modulate endoreduplication.SNW/SKI-INTERACTING PROTEIN interacts with UBIQUITIN-SPECIFIC PROTEASE14 and ULTRAVIOLET-B INSENSITIVE4 to influence endoreduplication and cell growth in Arabidopsis.