A self-organising biomimetic collagen/nano-hydroxyapatite-glycosaminoglycan scaffold for spinal fusion.
A self-organising biomimetic collagen/nano-hydroxyapatite-glycosaminoglycan scaffold for spinal fusion.
复制标题
用于脊柱融合的自组织仿生胶原蛋白/纳米羟基磷灰石-糖胺聚糖支架。
DOI：
10.1007/s10853-017-1229-9
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发表时间：
2017
影响因子：
4.5
通讯作者：
Czernuszka,Jan
中科院分区：
文献类型：
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作者：
Sharma,Aman;Brand,David;Fairbank,Jeremy;Ye,Hua;Lavy,Chris;Czernuszka,Jan
脊柱融合术作为治疗退行性脊椎疾病和慢性背痛的方法正在增加。然而，这项技术需要使用一种骨移植材料来融合椎骨，传统上是自体骨，它由成骨细胞前体、细胞外基质蛋白和矿物成分的最佳组合组成。到目前为止，这种材料仍然是“黄金标准”材料，但其供应有限，并与许多临床和伦理困难有关；因此，各种细胞与生物支架材料的组合已经进行了测试，但未能达到甚至可与自体骨相媲美的融合率。我们成功地将纳米羟基磷灰石和硫酸软骨素结合在一起，通过微生物转谷氨酰胺酶进行交联，成功地制备了一种新型的胶原基支架。利用一系列成像、化学成分和热分析技术对支架进行了表征。结果表明，MSCs具有合适的硬度和合适的孔径，有利于MSCs的黏附、生长和分化。低毒性使其适合临床应用，其缓慢的降解特性将使支架能够在较长时间内促进骨生长。因此，这种材料在脊柱融合和其他需要使用骨移植的程序中显示出临床应用的前景。
The use of spinal fusion surgery as a treatment for degenerative spinal conditions and chronic back pain is increasing. However, this technique requires use of a bone grafting material to fuse the vertebrae, traditionally autologous bone, which consists of an optimal combination of osteogenic cell precursors, extracellular matrix proteins and mineral components. To date, this remains the ‘gold standard’ material but its supply is limited and is associated with a number of clinical and ethical difficulties; consequently, various combinations of cells with biological scaffold materials have been tested but have failed to achieve fusion rates even comparable to autologous bone. We successfully fabricated a novel collagen-based scaffold using self-organising atelocollagen combined with nano-hydroxyapatite and chondroitin sulphate, cross-linked by microbial transglutaminase. The scaffold was characterised using a range of imaging, chemical composition and thermal analysis techniques. It was found to exhibit appropriate stiffness and suitable pore size for the adhesion, growth and differentiation of MSCs. The low toxicity makes it suitable for clinical application, and its slow degradation profile would enable the scaffold to promote bone growth over an extended period. This material therefore shows promise for clinical use in spinal fusion and other procedures requiring the use of bone grafts.