Global identification of miRNAs and targets in Populus euphratica under salt stress
Global identification of miRNAs and targets in Populus euphratica under salt stress
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盐胁迫下胡杨 miRNA 和靶标的整体鉴定
DOI：
10.1007/s11103-013-0010-y
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发表时间：
2013-04-01
影响因子：
5.1
通讯作者：
Xia, Xinli
中科院分区：
文献类型：
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作者：
Li, Bosheng;Duan, Hui;Xia, Xinli
胡杨是一种典型的水生盐生植物，是研究木本植物盐胁迫响应的理想材料。MicroRNAs(MiRNAs)是一种内源性非编码小RNA，具有重要的转录后调节功能。MiRNA可能调节对盐胁迫的耐受性，但这在胡杨中还没有被广泛研究。本研究对盐胁迫处理的胡杨的小RNA组、降解组和转录组进行了深度测序研究。在毛果杨和胡杨之间发现了211个保守的miRNA。此外，在胡杨中还发现了新的miRNAs，分属93个科162个。降解组测序实验验证了属于51个已鉴定miRNAs的112个靶标，其中很少有以前在胡杨中已知的miRNAs。转录组图谱显示，15对miRNA-靶基因在盐胁迫下的表达呈现反向变化。综上所述，这些结果表明，在盐胁迫下的胡杨中，可以发现大量新的miRNAs，并且已知的和新的miRNA都在功能上切割了它们的目标mRNA。盐胁迫相应地诱导了miRNA和靶基因的表达，但这是一个复杂的过程。
Populus euphratica, a typical hydro-halophyte, is ideal for studying salt stress responses in woody plants. MicroRNAs (miRNAs) are endogenous non-coding small RNAs that fulfilled an important post-transcriptional regulatory function. MiRNA may regulate tolerance to salt stress but this has not been widely studied in P. euphratica. In this investigation, the small RNAome, degradome and transcriptome were studied in salt stress treated P. euphratica by deep sequencing. Two hundred and eleven conserved miRNAs between Populus trichocarpa and P. euphratica have been found. In addition, 162 new miRNAs, belonging to 93 families, were identified in P. euphratica. Degradome sequencing experimentally verified 112 targets that belonged to 51 identified miRNAs, few of which were known previously in P. euphratica. Transcriptome profiling showed that expression of 15 miRNA-target pairs displayed reverse changing pattern under salt stress. Together, these results indicate that, in P. euphratica under salt stress, a large number of new miRNAs could be discovered, and both known and new miRNA were functionally cleaving to their target mRNA. Expression of miRNA and target were correspondingly induced by salt stress but that it was a complex process in P. euphratica.