The RPMI-1640 vitamin mixture promotes bovine blastocyst development in vitro and downregulates gene expression of TXNIP with epigenetic modification of associated histones
The RPMI-1640 vitamin mixture promotes bovine blastocyst development in vitro and downregulates gene expression of TXNIP with epigenetic modification of associated histones
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DOI：
10.1017/s2040174417000563
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发表时间：
2018-02-01
影响因子：
1.7
通讯作者：
Kume, S.
中科院分区：
文献类型：
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作者：
Ikeda, S.;Sugimoto, M.;Kume, S.
植入前胚胎周围的各种环境条件，包括可用的营养物质，会以短期和长期的方式影响其代谢和发育。硫氧还蛋白相互作用蛋白（TXNIP）是一个可能的标记植入前的压力，在体外受精（IVF）诱导的长期DOHaD的影响。B族维生素作为一碳代谢的参与者，可能通过代谢和发育重要基因的表观遗传改变影响植入前胚胎。在体外生产的牛胚胎培养或不与罗斯韦尔公园纪念研究所1640维生素混合物，含有B族维生素和B类维生素的物质，从体外受精后第3天，我们评估囊胚发育和TXNIP信使RNA（mRNA）的表达，在囊胚的逆转录-定量聚合酶链反应。用染色质免疫沉淀聚合酶链反应半定量地检测组蛋白H3赖氨酸27（H3 K27 me 3）在TXNIP启动子处的三甲基化程度。还通过蛋白质印迹分析比较各组之间的总H3 K27 me 3。维生素处理显著提高了第8天的囊胚发育率（P < 0.05）和囊胚孵化率（P < 0.001）。与对照组相比，维生素混合物处理组囊胚中TXNIP mRNA的表达降低（P < 0.01），同时其启动子H3 K27 me 3的表达水平升高（P < 0.05）。维生素混合物治疗组总H3 K27 me 3也高于对照组（P < 0.01）。在围受孕期的子宫内和/或体外营养条件下，与重要代谢过程相关的基因的表观遗传控制可能对这一时期的发育编程有可能影响农场动物的福利和生产性状。
Diverse environmental conditions surrounding preimplantation embryos, including available nutrients, affect their metabolism and development in both short-and long-term manner. Thioredoxin-interacting protein (TXNIP) is a possible marker for preimplantation stress that is implicated in in vitro fertilization- (IVF) induced long-term DOHaD effects. B vitamins, as participants in one-carbon metabolism, may affect preimplantation embryos by epigenetic alterations of metabolically and developmentally important genes. In vitro-produced bovine embryos were cultured with or without Roswell Park Memorial Institute 1640 vitamin mixture, containing B vitamins and B vitamin-like substances, from day 3 after IVF and we evaluated blastocyst development and TXNIP messenger RNA (mRNA) expression in the blastocysts by reverse transcription-quantitative polymerase chain reaction. The degree of trimethylation of histone H3 lysine 27 (H3K27me3) at TXNIP promoter was examined semi-quantitatively by chromatin immunoprecipitation polymerase chain reaction. Total H3K27me3 were also compared between the groups by Western blot analysis. The vitamin treatment significantly increased the rates of blastocyst development (P < 0.05) and their hatching (P < 0.001) from the zona pellucida by day 8. The mRNA expression of TXNIP was lower (P < 0.01) in blastocysts in the vitamin-mixture-treated group concomitant with higher (P < 0.05) level of H3K27me3 of its promoter compared with the control group. The total H3K27me3 in the vitamin-mixture-treated group was also higher (P < 0.01) than that in the control group. The epigenetic control of genes related to important metabolic processes during the periconceptional period by nutritional conditions in utero and/or in vitro may have possible implication for the developmental programming during this period that may impact the welfare and production traits of farm animals.