RAPID ACCUMULATION OF DELETED MITOCHONDRIAL DEOXYRIBONUCLEIC-ACID IN POSTMENOPAUSAL OVARIES
RAPID ACCUMULATION OF DELETED MITOCHONDRIAL DEOXYRIBONUCLEIC-ACID IN POSTMENOPAUSAL OVARIES
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DOI：
10.1095/biolreprod49.4.730
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发表时间：
1993-10-01
影响因子：
3.6
通讯作者：
TOMODA, Y
中科院分区：
文献类型：
--
作者：
KITAGAWA, T;SUGANUMA, N;TOMODA, Y
为了研究人卵巢的衰老过程，我们分析了不同年龄妇女卵巢线粒体DNA(MtDNA)突变的积累情况。在人工流产或妇科手术中收集了39个卵巢和腹肌组织，从胎儿的组织到77岁妇女的组织。提取组织总DNA，聚合酶链式反应扩增部分线粒体DNA。通过这些实验，我们发现在绝经和绝经后妇女的卵巢样本和所有肌肉样本中，ATPase8和ND5基因之间的mtDNA存在4977个碱基的缺失。线粒体DNA缺失的定量分析还表明，缺失的线粒体DNA在卵巢中的积累在绝经期前后迅速增加，而在腹部肌肉中的积累则随着年龄的增长而逐渐增加。这些结果表明，即使在很小的年龄，肌肉组织中也存在mtDNA的缺失，但卵巢组织中的缺失在绝经期就开始积累，并可能与年龄增长时卵巢功能障碍有关。
To study the aging process in human ovary, we analyzed the accumulation of mitochondrial DNA (mtDNA) mutation in ovary in women of various ages. Thirty-nine tissues from ovaries and abdominal muscles, ranging from those of a fetus to those of a 77-yr-old woman, were collected at artificial abortion or gynecological surgery. Total DNA was extracted from these tissues, and parts of mtDNA were amplified via polymerase chain reaction. Through these experiments, we found a 4977-bp deletion of mtDNA between the ATPase8 and ND5 genes in ovarian samples obtained from menopausal and postmenopausal women and in all muscle samples. Quantitative analysis of the deleted mtDNA also showed that the accumulation of deleted mtDNA in the ovary occurs rapidly around the menopausal period while the accumulation found in abdominal muscle occurs gradually with aging. These results indicated that the mtDNA deletion occurs in muscular tissues even at very young ages but that accumulation of the deletion in ovarian tissue starts at the menopausal period and may have a relationship to dysfunction of the ovary in aging.