EFFECT OF HEMORRHAGIC-SHOCK ON BACTERIAL TRANSLOCATION, INTESTINAL MORPHOLOGY, AND INTESTINAL PERMEABILITY IN CONVENTIONAL AND ANTIBIOTIC-DECONTAMINATED RATS
EFFECT OF HEMORRHAGIC-SHOCK ON BACTERIAL TRANSLOCATION, INTESTINAL MORPHOLOGY, AND INTESTINAL PERMEABILITY IN CONVENTIONAL AND ANTIBIOTIC-DECONTAMINATED RATS
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DOI：
10.1097/00003246-199005000-00014
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发表时间：
1990-05-01
影响因子：
8.8
通讯作者：
SPECIAN, RD
中科院分区：
文献类型：
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作者：
DEITCH, EA;MORRISON, J;SPECIAN, RD
细菌移位和回肠和盲肠损伤已被证明发生在有限的失血性休克后24小时。本研究旨在确定失血性休克后粘膜损伤、通透性和细菌移位的时间顺序。结果表明，细菌移位和粘膜损伤发生后2小时，30分钟的休克发作（平均动脉压30毫米汞柱）。虽然肠粘膜损伤的组织学程度在休克后2小时小于休克后24小时，在这两个时间肠屏障功能丧失辣根过氧化物酶的渗透性测定。由于移位细菌在休克后增强肠屏障功能丧失中的作用尚不清楚，第二个目标是确定休克诱导的粘膜损伤和通透性的程度是否可以通过肠道的抗生素去污来减少或消除。休克后2 h肠道植物群正常（> 106个细菌/g盲肠）的大鼠和无菌净化大鼠（< 103个细菌/g盲肠）的休克诱导的粘膜损伤和肠通透性的程度相似，尽管细菌移位的发生率分别为67%和0。因此，休克引起的粘膜渗透性和损伤似乎与易位细菌的存在没有直接关系。
Bacterial translocation and ileal and cecal injury have been shown to occur 24 h after limited periods of hemorrhagic shock. The present studies were performed to determine the temporal sequence of mucosal injury, permeability, and bacterial translocation after hemorrhagic shock. The results indicated that bacterial translocation and mucosal injury have occurred by 2 h after a 30-min episode of shock (mean arterial pressure 30 mm Hg). Although the histologic extent of the intestinal mucosal injury was less at 2 h postshock than at 24 h postshock, at both times intestinal barrier function was lost as measured by permeability to horseradish peroxidase. Since the role of translocating bacteria in potentiating the loss of intestinal barrier function after shock is unclear, the second goal was to determine whether the extent of shock-induced mucosal injury and permeability could be reduced or abrogated by antibiotic decontamination of the gut. The extent of shock-induced mucosal injury and intestinal permeability was similar between rats with a normal gut flora (> 106 bacteria/g cecum) and antibiotic-decontaminated rats (< 103 bacteria/g cecum) 2 h postshock, although the incidences of bacterial translocation were 67% and 0, respectively. Thus shock-induced mucosal permeability and injury appear not to be directly related to the presence of translocating bacteria.