The gut microbiome, short chain fatty acids, and related metabolites in cystic fibrosis patients with and without colonic adenomas.
The gut microbiome, short chain fatty acids, and related metabolites in cystic fibrosis patients with and without colonic adenomas.
复制标题
患有和不患有结肠腺瘤的囊性纤维化患者的肠道微生物组、短链脂肪酸和相关代谢物。
DOI：
10.1016/j.jcf.2023.01.013
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Abrams,JulianA
中科院分区：
文献类型：
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作者：
Baldwin-Hunter,BrittanyL;Rozenberg,FelixD;Annavajhala,MediniK;Park,Heekuk;DiMango,EmilyA;Keating,ClaireL;Uhlemann,Anne-Catrin;Abrams,JulianA
BackgroundAdults with cystic fibrosis (CF) are at increased risk for colon cancer. CF patients have reductions in intestinal bacteria that produce short chain fatty acids (SCFAs), although it is unclear whether this corresponds with intestinal SCFA levels and the presence of colonic neoplasia. The aim of this study was to compare gut microbiome and SCFA composition in patients with and without CF, and to assess associations with colonic adenomas.MethodsColonic aspirates were obtained from adults with and without CF undergoing colon cancer screening or surveillance colonoscopy. Microbiome characterization was performed by 16S rRNA V3-V4 sequencing. Targeted profiling of SCFAs and related metabolites was performed by LC-MS.Results42 patients (21 CF, 21 control) were enrolled. CF patients had significantly reduced alpha diversity and decreased relative abundance of many SCFA-producing taxa. There were no significant differences in SCFA levels in CF patients, although there were reduced levels of branched chain fatty acids (BCFAs) and related metabolites. CF patients with adenomas, but not controls with adenomas, had significantly increased relative abundance of Bacteroides fragilis. CF microbiome composition was significantly associated with isovalerate concentration and the presence of adenomas.ConclusionsCF patients have marked disturbances in the gut microbiome, and CF patients with adenomas had notably increased relative abundance ofB. fragilis, a pathogen known to promote colon cancer. Reductions in BCFAs but not SCFAs were found in CF. Further studies are warranted to evaluate the role ofB. fragilisas well the biological significance of reductions in BCFAs in CF.
