Measuring CaMKII concentration in dendritic spines.
Measuring CaMKII concentration in dendritic spines.
复制标题
测量树突状刺中的CaMKII浓度。
DOI：
10.1016/j.jneumeth.2011.09.022
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发表时间：
2012-01-15
影响因子：
3
通讯作者：
Lisman, John
中科院分区：
文献类型：
--
作者：
Otmakhov, Nikolai;Lisman, John
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Here, we present a method for measuring the concentration of endogenous protein in cellular compartments. Importantly, the method is applicable to compartments such as dendritic spines with dimensions often close to the resolution limit of optical microscopy. To our knowledge, a method with such capabilities has not yet been described. The method utilizes overexpression of the protein of interest, which is tagged with fluorescent protein. This is followed by immunostaining of both overexpressed and endogenous proteins. Expression of a volume marker is also required. We applied this method to measure the concentration of Ca/Calmodulin kinase II (CaMKII) in different cellular compartments of hippocampal pyramidal neurons. It was found that the concentrations of CaMKIIα subunits in cell bodies, proximal dendrites, and spines on these dendrites are 71, 46, and 103 μM, respectively. Considering the 3:1 ratio of α to β CaMKII subunits in the hippocampus, the concentrations of total (α + β) CaMKII subunits in these compartments are 94, 61, and 138 μM, respectively.
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影响因子：
64.8
作者：
Lee, Seok-Jin R.;Escobedo-Lozoya, Yasmin;Szatmari, Erzsebet M.;Yasuda, Ryohei
通讯作者：
Yasuda, Ryohei
影响因子：
5.3
作者：
Zhabotinsky, Anatol M.;Camp, R. Nicholas;Lisman, John E.
通讯作者：
Lisman, John E.
DOI：
10.1073/pnas.93.23.13250
发表时间：
1996-11-12
影响因子：
11.1
作者：
Mayford, M;Baranes, D;Kandel, ER
通讯作者：
Kandel, ER
影响因子：
6.2
作者：
Richards, HA;Halfhill, MD;Stewart, CN
通讯作者：
Stewart, CN
DOI：
10.1523/jneurosci.0133-10.2010
发表时间：
2010-06-30
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Pi HJ;Otmakhov N;Lemelin D;De Koninck P;Lisman J
通讯作者：
Lisman J