Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity.
Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity.
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DOI：
10.1016/j.actbio.2013.08.030
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发表时间：
2014
影响因子：
9.7
通讯作者：
M. Stevanović;Ines Bračko;M. Milenković;N. Filipović;Jana Nunić;M. Filipič;D. Uskoković
中科院分区：
文献类型：
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作者：
M. Stevanović;Ines Bračko;M. Milenković;N. Filipović;Jana Nunić;M. Filipič;D. Uskoković
将水溶性抗氧化剂(抗坏血酸、维生素C)与聚丙交酯-乙交酯共聚物(PLGA)包覆的银纳米粒子(AgNpPGA)包覆在一起，研究了它们的协同效应。采用溶剂/非溶剂体系物理化学法合成的PLGA/AgNpPGA/抗坏血酸粒子为球形，平均粒径为775 nm，粒径分布较窄，多分散指数为0.158。测定了AgNpPGA/抗坏血酸在PLGA中的包封率为90%。囊化抗坏血酸在68天内全部释放，AgNpPGAs在87天内降解释放。研究了PLGA/AgNpPGA/抗坏血酸对细胞活力、细胞内活性氧(ROS)生成以及对7种不同病原菌的抑菌活性的影响。3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium溴化实验表明，这些聚乳酸/银/抗坏血酸具有良好的生物相容性。我们用DCFH-DA法测定了HepG2细胞中ROS的形成动力学，发现PLGA/AgNpPGA/抗坏血酸在暴露5h后引起DCF荧光强度显著下降，是对照组的2倍。这表明PLGA/AgNpPGA/抗坏血酸微球要么作为细胞内ROS的清除剂和/或减少它们的形成。此外，用肉汤微量稀释法得到的PLGA/AgNpPGA/抗坏血酸的抑菌活性结果表明，这些粒子具有良好的抗菌活性和延伸性。用傅里叶变换红外光谱、场发射扫描电子显微镜、透射电子显微镜、Zeta电位和粒度分析对样品进行了表征。本文提出了一种治疗感染的新方法，同时提供了非常显著的抗氧化效果。
A water-soluble antioxidant (ascorbic acid, vitamin C) was encapsulated together with poly(l-glutamic acid)-capped silver nanoparticles (AgNpPGA) within a poly(lactide-co-glycolide) (PLGA) polymeric matrix and their synergistic effects were studied. The PLGA/AgNpPGA/ascorbic acid particles synthesized by a physicochemical method with solvent/non-solvent systems are spherical, have a mean diameter of 775 nm and a narrow size distribution with a polydispersity index of 0.158. The encapsulation efficiency of AgNpPGA/ascorbic acid within PLGA was determined to be >90%. The entire amount of encapsulated ascorbic acid was released in 68 days, and the entire amount of AgNpPGAs was released in 87 days of degradation. The influence of PLGA/AgNpPGA/ascorbic acid on cell viability, generation of reactive oxygen species (ROS) in HepG2 cells, as well as antimicrobial activity against seven different pathogens was investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGA/ascorbic acid particles. We measured the kinetics of ROS formation in HepG2 cells by a DCFH-DA assay, and found that PLGA/AgNpPGA/ascorbic acid caused a significant decrease in DCF fluorescence intensity, which was 2-fold lower than that in control cells after a 5 h exposure. This indicates that the PLGA/AgNpPGA/ascorbic acid microspheres either act as scavengers of intracellular ROS and/or reduce their formation. Also, the results of antimicrobial activity of PLGA/AgNpPGA/ascorbic acid obtained by the broth microdilution method showed superior and extended activity of these particles. The samples were characterized using Fourier transform infrared spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, zeta potential and particle size analysis. This paper presents a new approach to the treatment of infection that at the same time offers a very pronounced antioxidant effect.