Sequence analysis of a Kpnl family member near the 3′ end of human β-globin gene
Sequence analysis of a Kpnl family member near the 3′ end of human β-globin gene
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人β-珠蛋白基因3′端附近Kpnl家族成员的序列分析
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
Y. Sakaki
中科院分区：
文献类型：
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作者：
M. Hattori;S. Hidaka;Y. Sakaki
我们测定了人Kpn I家族的全长成员T β G41的完整核苷酸序列（6125 bp），其位于β-珠蛋白基因下游约3 kb处。将该序列与Singer编制的Kpn I家族序列进行比较，发现T β G41中存在一个新的131 bp序列。杂交分析表明，几千名人类Kpn I家族成员携带这种额外的序列。计算机检索DNA数据库中的T β G41同源序列发现，一些T β G41同源序列与假基因密切相关。T β G41序列与鸡转铁蛋白样转化基因ChBlym-1也有很高的同源性。此外，从T β G41核苷酸序列推导的氨基酸序列显示与人转铁蛋白和乳转铁蛋白的氨基酸序列具有相对高的同源性。
We determined the complete nucleotide sequence (6125 bp) of a full-length member of human KpnI family, designated T beta G41, which is located about 3 kb downstream from the beta-globin gene. Comparison of the sequence with the KpnI family sequence compiled by Singer revealed that a new 131 bp sequence is present in the T beta G41. Hybridization analyses showed that a few thousand of human KpnI family members are carrying this additional sequence. Computer search of DNA databases for T beta G41-homologous sequence showed that some T beta G41-homologous sequences were closely associated with pseudogenes. The T beta G41 sequence also showed significant sequence homology with ChBlym-1, a transferrin-like transforming gene of chicken. Furthermore, an amino acid sequence deduced from the T beta G41 nucleotide sequence revealed a relatively-high homology to those of human transferrin and lactotransferrin.