Enzymatically triggered shape memory polymers
Enzymatically triggered shape memory polymers
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DOI：
10.1016/j.actbio.2018.11.031
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发表时间：
2019-01-15
影响因子：
9.7
通讯作者：
Henderson, James H.
中科院分区：
文献类型：
--
作者：
Buffington, Shelby L.;Paul, Justine E.;Henderson, James H.
由热或光热触发激活的细胞相容性形状记忆聚合物已被开发并建立为基础和转化研究的强大的“智能材料”平台。相比之下，尚未报道可由生物活性直接触发的形状记忆聚合物（SMP）。本研究的目的是开发一种SMP，直接响应酶活性，并可以在等温细胞培养条件下这样做。为了实现这一目标，我们设计了一种SMP，其具有易受酶促降解的形状固定组分聚（ε-己内酯）（PCL）和酶促稳定的形状记忆组分Pellethane-当形状固定组分经历酶促降解时，SMP返回到其原始的编程形状。我们定量和定性分析了酶催化形状记忆反应的材料特性、形状记忆性能和细胞相容性。结果表明，酶的恢复，作为拉伸试样的收缩，使用大量的酶降解实验，并表明形状恢复是通过降解的PCL形状固定相。结果进一步表明，材料和酶促形状恢复的过程都是细胞相容的。因此，这里报道的SMP设计代表了能够施加程序化形状变化或直接机械力的酶响应材料和可以直接响应于生物活性的SMP。SignificanceCytocompatible形状记忆聚合物由热或光热触发器激活已成为基础和转化研究的强大的“智能材料”平台。相比之下，还没有报道可以通过生物活性直接触发的形状记忆聚合物。在这里，我们报告了酶促触发的形状记忆聚合物，改变其形状等温响应酶的活性。我们成功地证明了酶的恢复使用散装酶降解实验，并表明形状恢复是通过降解的形状固定相。我们进一步表明，材料和酶促形状恢复的过程是细胞相容的。这种新的形状记忆聚合物设计可以预期在基础和应用材料科学中作为刺激响应材料实现新的应用。(C)2018 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Cytocompatible shape memory polymers activated by thermal or photothermal triggers have been developed and established as powerful "smart material" platforms for both basic and translational research. Shape memory polymers (SMPs) that could be triggered directly by biological activity have not, in contrast, been reported. The goal of this study was to develop an SMP that responds directly to enzymatic activity and can do so under isothermal cell culture conditions. To achieve this goal, we designed an SMP with a shape fixing component, poly(epsilon-caprolactone) (PCL), that is vulnerable to enzymatic degradation and a shape memory component, Pellethane, that is enzymatically stable - as the shape fixing component undergoes enzymatically-catalyzed degradation, the SMP returns to its original, programmed shape. We quantitatively and qualitatively analyzed material properties, shape memory performance, and cytocompatibility of the enzymatically-catalyzed shape memory response. The results demonstrate enzymatic recovery, as contraction of tensile specimens, using bulk enzymatic degradation experiments and show that shape recovery is achieved by degradation of the PCL shape-fixing phase. The results further showed that both the materials and the process of enzymatic shape recovery are cytocompatible. Thus, the SMP design reported here represents both an enzyme responsive material capable of applying a programmed shape change or direct mechanical force and an SMP that could respond directly to biological activity.Statement of SignificanceCytocompatible shape memory polymers activated by thermal or photothermal triggers have become powerful "smart material" platforms for basic and translational research. Shape memory polymers that could be triggered directly by biological activity have not, in contrast, been reported. Here we report an enzymatically triggered shape memory polymer that changes its shape isothermally in response to enzymatic activity. We successfully demonstrate enzymatic recovery using bulk enzymatic degradation experiments and show that shape recovery is achieved by degradation of the shape-fixing phase. We further show that both the materials and the process of enzymatic shape recovery are cytocompatible. This new shape memory polymer design can be anticipated to enable new applications in basic and applied materials science as a stimulus responsive material. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.