Cell Growth-Promoting Activity of Fluid from Eye Sacs of the Bubble-Eye Goldfish (Carassius auratus)
Cell Growth-Promoting Activity of Fluid from Eye Sacs of the Bubble-Eye Goldfish (Carassius auratus)
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DOI：
10.2108/zsj.26.254
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发表时间：
2009-04-01
影响因子：
0.9
通讯作者：
Wakamatsu, Yuko
中科院分区：
文献类型：
--
作者：
Sawatari, Etsuko;Hashimoto, Hisashi;Wakamatsu, Yuko
鱼类体液，如血清和胚胎提取物，对鱼类细胞培养的促进生长作用已被广泛证明。水族馆金鱼的特征是每只眼睛下面都有一个充满液体(囊液)的大囊。这些囊被认为含有淋巴液，其成分与血清或血浆相似。为了验证囊液能否作为生长因子补充剂中胎牛血清(FBS)的添加剂，我们比较了细胞在含胎牛血清的培养液和不同浓度的囊液中的生长情况。在青冈鱼和斑马鱼的早期传代尾鳍细胞中观察到了剂量依赖的促生长效应。在早期传代的青竹胚泡细胞和斑马鱼胚胎细胞系(ZF4)中也证实了促进细胞生长的作用。在收集囊液后的几个月内，泡眼患者眼囊内的液体就会被替换，而新液体的促进细胞生长的活性与最初采集的液体相似。这些发现表明，囊液可以作为鱼类细胞培养的促生长添加剂。重要的是，金鱼能够替代液体，再加上等量的液体可以从眼囊中反复采集，这意味着可以在不牺牲鱼类的情况下获得可持续的液体来源。
The growth-promoting effects of fish body fluids, such as serum and embryonic extract, on fish cell cultures have been widely demonstrated. The bubble-eye variety of aquarium goldfish is characterized as having a large sac filled with fluid (sac fluid) under each eye. These sacs are believed to contain lymph, which is similar in composition to serum or blood plasma. In order to test whether the sac fluid can be used as an additive for fetal bovine serum (FBS) in growth factor supplements, we compared cell growth in media containing FBS together with different concentrations of sac fluid. A dose-dependent growth-promotion effect was observed in early passage caudal fin cells from both medaka and zebrafish. Cell-growth promotion was also confirmed in early passage medaka blastula cells and in a zebrafish embryonic cell line (ZF4). Replacement of the fluid in the eye sacs of bubble-eyes occurs within a couple of months after the sac fluid has been harvested, and the cell-growth promoting activity of the new fluid is similar to that of the fluid that was tapped initially. These findings suggest that sac fluid can be used as a growth-promoting supplement for fish cell culture. Importantly, the ability of the goldfish to replace the fluid combined with the fact that equipotent fluid can be repeatedly harvested from the eye sacs means that a sustainable source of the fluid can be obtained without needing to sacrifice the fish.