Three-dimensional imaging of whole rodent organs using optical computed and emission tomography
Three-dimensional imaging of whole rodent organs using optical computed and emission tomography
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DOI：
10.1117/1.2709858
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发表时间：
2007-01-01
影响因子：
3.5
通讯作者：
Dewhirst, Mark
中科院分区：
文献类型：
--
作者：
Oldham, Mark;Sakhalkar, Harshad;Dewhirst, Mark
我们探索光学计算机断层扫描 (optical-CT) 和光学发射计算机断层扫描 (optical-ECT) 在新的区域全器官成像中的潜力。这些技术在内部原型台式系统上实施，与以前基于体视显微镜的系统相比，该系统具有更高的图像质量和对更大样品（最大 3 厘米）成像的能力。成像性能测试证实了高几何精度、精确的线性衰减系数相对测量以及 50 μm 级别特征成像的能力。使用通过自然体内循环过程沉积的两种染色剂实现器官微脉管系统的光学标记：被动吸收墨水基染色剂和活性荧光素 FITC-凝集素缀合物。凝集素蛋白与内皮细胞壁结合，FITC 荧光可实现光学 ECT 成像。已获取正常大鼠心脏、左肺和小鼠右肺的三维 (3-D) 光学 CT 图像，显示功能性脉管系统和相对灌注分布的精美细节。还获得了小鼠肺和肾的配准光学 ECT 图像。组织学切片证实了整个器官的微脉管系统的有效标记。光学 CT 和光学 ECT 的优点包括具有高分辨率和高对比度的独特组合的潜力，以及与多种光学探针（包括基因表达标记荧光报告蛋白）的兼容性。 (c) 2007 年光电仪器工程师协会。
We explore the potential of optical computed tomography (optical-CT) and optical emission computed tomography (optical-ECT) in a new area-whole organ imaging. The techniques are implemented on an in-house prototype benchtop system with improved image quality and the capacity to image larger samples (up to 3 cm) than previous systems based on stereo microscopes. Imaging performance tests confirm high geometrical accuracy, accurate relative measurement of linear attenuation coefficients, and the ability to image features at the 50-mu m level. Optical labeling of organ microvasculature was achieved using two stains deposited via natural in vivo circulatory processes: a passive absorbing ink-based stain and an active fluorescin FITC-lectin conjugate. The lectin protein binds to the endothelial lining, and FITC fluorescense enables optical-ECT imaging. Three-dimensional (3-D) optical-CT images have been acquired of a normal rat heart and left lung and a mouse right lung showing exquisite detail of the functional vasculature and relative perfusion distribution. Coregistered optical-ECT images were also acquired of the mouse lung and kidney. Histological sections confirmed effective labeling of microvasculature throughout the organs. The advantages of optical-CT and optical-ECT include the potential for a unique combination of high resolution and high contrast and compatibility with a wide variety of optical probes, including gene expression labeling fluorescent reporter proteins. (c) 2007 Society of Photo-Optical Instrumentation Engineers.