Identification and characterization of drought-tolerant local pigmented rice from Indonesia
Identification and characterization of drought-tolerant local pigmented rice from Indonesia
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印度尼西亚耐旱本地色素稻的鉴定与表征
DOI：
10.1007/s12298-022-01185-5
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发表时间：
2022
影响因子：
3.5
通讯作者：
Purwestri Yekti Asih
中科院分区：
文献类型：
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作者：
Sebastian Alfino;Nugroho Ilham Cahyo;Putra Herdin Surya Dwi;Susanto Febri Adi;Wijayanti Putri;Yamaguchi Nobutoshi;Nuringtyas Tri Rini;Purwestri Yekti Asih
水是维持生命所必需的。由于有限的水分供应可能会影响它们的生命周期，植物对干旱胁迫产生了多种反应。干旱期间植物生理和代谢的变化可能反映了基因表达水平的变化。在这项研究中，我们调查了色素水稻品种的抗旱策略的变化及其可能的耐旱性相关基因。我们筛选了21个地方色素水稻品种从印度尼西亚增加耐旱性的部分蒸腾土壤水分的方法施加精确的控制施加在植物上的干旱胁迫。然后，我们确定了OsDREB1A、OsNAC6、OsNHX 1、OsCuZnSOD 2、OsOSCAT2和OsCAT 3在生长在良好水分条件下和中度或重度干旱胁迫下的植物中的表达。在色素水稻品种中，Merah Pari Eja的耐旱性最强，而红米Inpari 24的死亡率最高（60%）。我们还包括白色稻栽培品种Putih Payo，其对干旱完全敏感（在所用条件下具有100%死亡率）作为阴性对照。基因表达谱分析显示，普遍上调的干旱相关基因的Merah Pari Eja和下调，这些基因在其他两个品种。抗氧化酶活性，叶片损伤，自由基，叶绿素和花青素含量的测量提供了进一步的证据，Merah Pari Eja比其他两个品种更耐旱。OsDREB1A、OsNAC6、OsNHX1、OsCuZnSOD 2、OsOSCAT2和OsCAT 3的表达模式可以揭示植物具有增强的耐旱性。
Water is essential to support life. Because limited water availability may affect their life cycles, plants have developed multiple responses to drought stress. Plant physiological and metabolic changes during drought may reflect changes that occur at the level of gene expression. In this study, we investigated the variation in drought-mitigating strategies employed by pigmented rice (Oryza sativa) varieties and the genes involved in their possible drought tolerance. We screened 21 local pigmented rice cultivars from Indonesia for increased drought tolerance using the fraction transpirable soil water method to exert precise control of the drought stress imposed on plants. We then determined the expression ofOsDREB1A,OsNAC6,OsNHX1,OsCuZnSOD2,OsOSCAT2, andOsCAT3in plants grown under well-watered conditions and under moderate or severe drought stress. Among the pigmented rice cultivars, Merah Pari Eja had the greatest drought tolerance, while the red rice Inpari 24 had the highest mortality rate (60%). We also included the white rice cultivar Putih Payo, which is fully sensitive to drought (with 100% mortality under the conditions used) as a negative control. Gene expression profiling revealed a general upregulation of drought-related genes in Merah Pari Eja and a downregulation of such genes in the other two cultivars. Measurements of antioxidant enzyme activity, leaf damage, free radicals, chlorophyll, and anthocyanin contents provided further evidence that Merah Pari Eja is more drought tolerant than the other two cultivars. We conclude thatOsDREB1A,OsNAC6,OsNHX1,OsCuZnSOD2,OsOSCAT2andOsCAT3expression patterns can reveal plants that have increased drought tolerance.