Dopamine receptor blockade increases glandular kallikrein-like activity in the neurointermediate lobe of the rat pituitary.
Dopamine receptor blockade increases glandular kallikrein-like activity in the neurointermediate lobe of the rat pituitary.
复制标题
多巴胺受体阻断可增加大鼠垂体神经中叶的腺激肽释放酶样活性。
DOI：
10.1016/s0006-291x(85)80213-8
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
Powers,CA
中科院分区：
文献类型：
--
作者：
Powers,CA
多巴胺受体阻滞剂对大鼠垂体神经中间叶腺激肽释放酶样活性的影响进行了研究。雄性大鼠每天注射氟哌啶醇（2.5 mg/kg）、奋乃静（5 mg/kg）或舒必利（60 mg/kg），连续7天。制备神经中间叶匀浆。用胰蛋白酶活化潜伏蛋白酶，并在37°C，pH 8.0下使用显色肽底物测量蛋白水解活性。所有三种多巴胺受体阻滞剂都使腺激肽释放酶样活性增加约100%。结果表明，腺激肽释放酶样活性在神经中间叶的多巴胺能机制的抑制控制下。
The effect of dopamine receptor blockers on glandular kallikrein-like activity in the neurointermediate lobe of the rat pituitary was examined. Male rats were given daily injections of haloperidol (2.5 mg/kg), perphenazine (5 mg/kg) or sulpiride (60 mg/kg) for 7 days. Homogenates of the neurointermediate lobe were prepared. Latent proteases were activated with trypsin and proteolytic activity was measured at 37°C, pH 8.0 using chromogenic peptide substrates. All three dopamine receptor blockers produced about a 100% increase in glandular kallikrein-like activity. The results suggest that glandular kallikrein-like activity in the neurointermediate lobe is under inhibitory control by dopaminergic mechanisms.