Starvation causes female-to-male sex reversal through lipid metabolism in the teleost fish, medaka (Olyzias latipes)
Starvation causes female-to-male sex reversal through lipid metabolism in the teleost fish, medaka (Olyzias latipes)
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DOI：
10.1242/bio.050054
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发表时间：
2020-04-01
期刊：
影响因子：
2.4
通讯作者：
Tanaka, Minoru
中科院分区：
文献类型：
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作者：
Sakae, Yuta;Oikawa, Akira;Tanaka, Minoru
硬骨鱼青鳉 (Oryzias latipes) 采用 XX/XY 遗传性别决定系统。我们在这里表明，青鳉的表型性别受到脂质代谢变化的影响。饥饿 5 天的青鳉幼虫经历了雌雄逆转。代谢组学和 RT-qPCR 分析表明，饥饿抑制了泛酸代谢。一致地，抑制泛酸代谢途径会导致性逆转。该途径的最终代谢物是辅酶 A，它是脂肪生成的重要因素。抑制脂肪酸合成（脂肪生成的第一步）也会导致性逆转。雄性发育的关键基因 dmrt1 的表达受到饥饿的抑制，并且 dmrt1 (Delta 13) 突变体在饥饿下没有表现出性逆转。总的来说，这些结果表明脂肪酸合成通过饥饿下雄性基因 dmrt1 的异位表达参与雌性向雄性的性别逆转。
The teleost fish, medaka (Oryzias latipes), employs the XX/XY genetic sex determination system. We show here that the phenotypic sex of medaka is affected by changes in lipid metabolism. Medaka larvae subjected to 5 days of starvation underwent female-to-male sex reversal Metabolomic and RT-qPCR analyses indicated that pantothenate metabolism was suppressed by starvation. Consistently, inhibiting the pantothenate metabolic pathway caused sex reversal. The final metabolite in this pathway is coenzyme A, an essential factor for lipogenesis. Inhibiting fatty acid synthesis, the first step of lipogenesis, also caused sex reversal. The expression of dmrt1, a critical gene for male development, was suppressed by starvation, and a dmrt1 (Delta 13) mutant did not show sex reversal under starvation. Collectively, these results indicate that fatty acid synthesis is involved in female-to-male sex reversal through ectopic expression of male gene dmrt1 under starvation.