Paraoxonase activity in high-density lipoproteins: A comparison between healthy and obese females
Paraoxonase activity in high-density lipoproteins: A comparison between healthy and obese females
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DOI：
10.1210/jc.2004-0486
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发表时间：
2005-03-01
影响因子：
5.8
通讯作者：
Bicchiega, V
中科院分区：
文献类型：
--
作者：
Ferretti, G;Bacchetti, T;Bicchiega, V
对氧磷酶是一种与高密度脂蛋白相关的酶，对循环细胞和脂蛋白的氧化损伤具有保护作用，调节高密度脂蛋白对糖基化、同型半胱氨酸化等致动脉粥样硬化的易感性，甚至发挥抗炎作用。本研究旨在探讨健康体检者和肥胖者脂蛋白氧化应激与高密度脂蛋白-过氧化物酶活性的关系。因此，比较肥胖女性(n=12)和年龄性别匹配的对照组(n=31)血浆中高密度脂蛋白和低密度脂蛋白的高密度脂蛋白-过氧化物酶活性和过氧化脂质水平。我们的结果首次显示肥胖者的高密度脂蛋白-PON活性显著低于对照组(P<；0.001)。此外，我们的结果显示，肥胖者的高密度脂蛋白和低密度脂蛋白中的过氧化脂质水平显著增加(P<；0.001)。与高密度脂蛋白和低密度脂蛋白相关的脂质过氧化水平与高密度脂蛋白-过氧化氢酶活性之间的负相关证实了对氧磷酶活性与脂蛋白的脂质过氧化之间的关系。肥胖者血浆瘦素水平与高密度脂蛋白-PON活性呈负相关，与高密度脂蛋白和低密度脂蛋白中过氧化脂质水平呈正相关，提示瘦素与脂蛋白氧化损伤有关。总之，我们的研究表明，肥胖者低密度脂蛋白和高密度脂蛋白中氧化应激的增加与高密度脂蛋白-PON活性的降低有关。对氧磷酶活性较低以及高密度脂蛋白和低密度脂蛋白的成分变化可能导致与肥胖相关的心血管疾病的更大风险。
Paraoxonase, an enzyme associated with high-density lipoprotein (HDL-PON), exerts a protective effect against oxidative damage of circulating cells and lipoproteins, modulates the susceptibility of HDL to atherogenic modifications such as glycation and homocysteinylation, and even exerts an antiinflammatory role. The aim of the present study was to investigate the relationship between lipoprotein oxidative stress and the activity of HDL-PON in healthy and obese subjects. Therefore, the activity of HDL-PON and the levels of lipid hydroperoxides in HDL and low-density lipoprotein (LDL) isolated from plasma of obese females ( n = 12) and age-sex-matched controls ( n = 31) were compared. Our results demonstrated for the first time that the activity of HDL-PON in obese subjects was significantly lower compared with that in controls ( P < 0.001). Moreover, our results showed a significant increase in the levels of lipid hydroperoxides in HDL and LDL isolated from obese subjects ( P< 0.001). The negative correlations established between HDL-PON activity and the levels of lipid hydroperoxides associated with HDL and LDL confirm the relationship between paraoxonase activity and lipid peroxidation of lipoproteins. Plasma levels of leptin correlated negatively with HDL-PON activity and positively with levels of lipid hydroperoxides in HDL and LDL of obese subjects, suggesting a relationship between leptin and oxidative damage of lipoproteins. In conclusion, our study demonstrated that the increase in oxidative stress in LDL and HDL of obese subjects is associated with a decrease in HDL-PON activity. The lower paraoxonase activity and the compositional changes in HDL and LDL could contribute to the greater risk of cardiovascular disease associated with obesity.