Synthesis, characterization and immobilization of Prussian blue nanoparticles.: A potential tool for biosensing devices
Synthesis, characterization and immobilization of Prussian blue nanoparticles.: A potential tool for biosensing devices
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DOI：
10.1039/b412583e
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发表时间：
2005-01-01
影响因子：
4.9
通讯作者：
de Torresi, SIC
中科院分区：
文献类型：
--
作者：
Fiorito, PA;Gonçales, VR;de Torresi, SIC
普鲁士蓝（PB）颗粒的大小约。5 nm的合成和固定在一个多层结构，作为一种战略的潜在发展的电流型传感器氧化酶-酶为基础的生物传感器。采用逐层（LbL）顺序沉积法制备了PB和聚烯丙胺盐酸盐（PAH）的多层膜。通过紫外可见光谱和循环伏安法仔细监测该过程。在逐层沉积过程中氧化还原电流峰的增加表明在膜内发生电荷传播。随着沉积的双层的数量的线性增加的紫外-可见吸收表明，组织良好的系统已经制定。用PB/PAH膜修饰的ITO电极成功地用于检测H_2O_2，灵敏度与PB/PAH膜的层数有关。
Prussian blue (PB) particles with the size of ca. 5 nm were synthesized and immobilized in a multilayer structure, as a strategy for the potential development of an amperometric transducer for oxidase-enzyme-based biosensors. Multilayer films composed of PB and poly( allylamine hydrochloride) (PAH) were prepared via layer-by-layer (LbL) sequential deposition. The process was carefully monitored by UV-vis spectroscopy and cyclic voltammetry. The increase of the redox current peaks during the layer-by-layer deposition demonstrated that charge propagation within the film occurs. Linear increase of UV-vis absorbance with the number of deposited bilayers indicates that well-organized systems have been elaborated. ITO electrodes coated with PB/PAH films were used successfully for detecting H2O2, sensitivity being dependent on the number of PB/PAH layers.