Efficacy of a DNA vaccine delivered in attenuated Salmonella typhimurium against Eimeria tenella infection in chickens
Efficacy of a DNA vaccine delivered in attenuated Salmonella typhimurium against Eimeria tenella infection in chickens
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DOI：
10.1016/j.ijpara.2005.03.005
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发表时间：
2005-06-01
影响因子：
4
通讯作者：
Wang, SH
中科院分区：
文献类型：
--
作者：
Du, AF;Wang, SH
在一项实验攻击研究中，检测了携带柔嫩艾美耳球虫5401抗原基因的口服DNA疫苗的效力。将重组质粒pcDNA3-5401转化减毒鼠伤寒沙门氏菌(Dam(-)和Phop(-))(以下简称ZJ111/pcDNA3-5401)，制备DNA疫苗。将鸡随机分为6组，每组50只。A组给予PBS作为对照。B组饲喂10(8)个减毒鼠伤寒沙门氏菌集落形成单位(CFU)。携带pcDNA3。C组肌肉注射100mg5401重组蛋白。D~F组分别口服ZJ111/pcDNA3-5401，每只鸡10(7)、10(8)、10(9)CFU。两周后，所有免疫接种均加强。在第二次免疫后14天，用6×10(4)同源孢子化卵囊攻击免疫鸡。在加强免疫前和加强免疫后4周，两组之间的体重没有显著差异。在免疫后6周，ZJ111/pcDNA3-5401最终从脾和肝中清除。经100代无抗生素培养后，在80%以上的减毒鼠伤寒沙门氏菌中保持稳定。ZJ111/pcDNA3-5401口服免疫鸡可引起特异性体液反应，并刺激外周血淋巴细胞增殖。各免疫组的淋巴细胞增殖反应均显著高于对照组。C组的抗体应答明显低于ZJ111/pcDNA3-5401免疫组。10(8)只(E组)和10(9)只(F组)接种ZJ111/pcDNA3-5401疫苗，对柔嫩艾美耳球虫攻击的保护率分别为55.0%和57.5%。这些结果对利用细菌载体口服递送系统研制禽球虫病DNA疫苗具有重要意义。(C)2005澳大利亚寄生虫学会有限公司出版。保留所有权利。
The efficacy of an oral DNA vaccine carrying the Eimeria tenella 5401 antigen gene delivered by attenuated Salmonella typhimurium was examined in an experimental challenge study. The DNA vaccine preparation was made by transforming the recombinant plasmid pcDNA3-5401 into the attenuated S. typhimurium strain (Dam(-) and PhoP(-)) (designated hereafter as ZJ111/pcDNA3-5401). The chickens were randomly divided into six groups, 50 per group. Group A were given PBS as control. Chickens in group B were fed with 10(8) colony forming units (CFU) of attenuated S. typhimurium. carrying pcDNA3. Group C were immunised with 100 mu g of the recombinant 5401 protein via intramuscular injection. Groups D to F orally received ZJ111/pcDNA3-5401 at doses of 10(7), 10(8) and 10(9) CFU per chicken, respectively. All immunisations were boosted 2 weeks later. The immunised chickens were challenged with 6 x 10(4) homologous sporulated oocysts 14 days after the second immunisation. No significant differences in body weight were detected between the groups before immunisation and at week 4 after the booster immunisation. The ZJ111/pcDNA3-5401 was eventually eliminated from the spleen and liver on week 6 post-immunisation. The plasmid pcDNA3-5401 was stably maintained in over 80% of the attenuated S. typhimurium population after 100 generations of growth in antibiotic-free media. Oral immunisation of chickens with ZJ111/pcDNA3-5401 elicited specific humoral responses and stimulated proliferation of peripheral blood lymphocytes. The lymphocyte proliferation response was significantly higher in all vaccinated groups than in the control chickens. Antibody response was significantly lower in group C than in groups imummised with strain ZJ111/pcDNA3-5401. Vaccination with the strain ZJ111/pcDNA3-5401 at 10(8) (group E) and 10(9) (group F) CFU per chicken provided 55.0 and 57.5% protection against E. tenella challenge, respectively. These results have important implications for the development of DNA vaccines against avian coccidiosis by bacteria-vectored oral delivery system. (c) 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.