Feruloylated and Nonferuloylated Arabino-oligosaccharides from Sugar Beet Pectin Selectively Stimulate the Growth of Bifidobacterium spp. in Human Fecal in Vitro Fermentations
Feruloylated and Nonferuloylated Arabino-oligosaccharides from Sugar Beet Pectin Selectively Stimulate the Growth of Bifidobacterium spp. in Human Fecal in Vitro Fermentations
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DOI：
10.1021/jf200996h
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发表时间：
2011-06-22
影响因子：
6.1
通讯作者：
Meyer, Anne S.
中科院分区：
文献类型：
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作者：
Holck, Jesper;Lorentzen, Andrea;Meyer, Anne S.
甜菜果胶中鼠李糖半乳糖醛酸 I 部分的侧链特别富含阿拉伯聚糖部分，这些部分可以被阿魏酰基取代。在这项工作中，通过 Amberlite XAD 疏水相互作用和膜分离，根据阿魏酰基取代和阿拉伯低聚糖链长，将基于果胶生产的甜菜浆产生的富含阿拉伯聚糖的级分分离为四个级分：分别是短链 (DP2-10) 和长链 (DP 7-14) 阿魏酰化和非阿魏酰化阿拉伯低聚糖。组分的 HPAEC、SEC 和 MALDI-TOF/TOF 分析证实阿魏酰取代组分中存在单取代和双取代的阿魏酰化阿拉伯寡糖。人类粪便样本（n = 6 名健康人类志愿者）的体外微生物发酵显示，阿魏酰化和非阿魏酰化长链阿拉伯低聚糖对双歧杆菌有选择性刺激，其程度与益生元低聚果糖对照相同。在单一培养物中，没有任何组分刺激潜在病原体艰难梭菌的生长。这项工作提供了关于从甜菜浆中分离潜在生物活性阿魏酰化阿拉伯低聚糖的第一份报告，并初步表明相对长链阿拉伯低聚糖相对于短链阿拉伯低聚糖具有潜在更大的双歧杆菌效应。
The side chains of the rhamnogalacturonan I fraction in sugar beet pectin are particularly rich in arabinan moieties, which may be substituted with feruloyl groups. In this work the arabinan-rich fraction resulting from sugar beet pulp based pectin production was separated by Amberlite XAD hydrophobic interaction and membrane separation into four fractions based on feruloyl substitution and arabino-oligosaccharide chain length: short-chain (DP2-10) and long-chain (DP 7-14) feruloylated and nonferuloylated arabino-oligosaccharides, respectively. HPAEC, SEC, and MALDI-TOF/TOF analyses of the fractions confirmed the presence of singly and doubly substituted feruloylated arabino-oligosaccharides in the feruloyl-substituted fractions. In vitro microbial fermentation by human fecal samples (n =6 healthy human volunteers) showed a selective stimulation of bifidobacteria by both the feruloylated and the nonferuloylated long-chain arabino-oligosaccharides to the same extent as the prebiotic fructo-oligosaccharides control. None of the fractions stimulated the growth of the potential pathogen Clostridium difficile in monocultures. This work provides a first report on the separation of potentially bioactive feruloylated arabino-oligosaccharides from sugar beet pulp and an initial indication of the potentially larger bifidogenic effect of relatively long-chain arabino-oligosaccharides as opposed to short-chain arabino-oligosaccharides.