The plant oncogene rolD stimulates flowering in transgenic tobacco plants.
The plant oncogene rolD stimulates flowering in transgenic tobacco plants.
复制标题
植物癌基因 rolD 刺激转基因烟草植物开花。
作者：
M. L. Mauro;M. Trovato;A. Paolis;A. Gallelli;P. Costantino;M. Altamura
将发根农杆菌T-DNA癌基因Rold在其自身的5‘调控区控制下，转移到日中和烟草植株中。在转基因植株中，Rold诱导的主要性状是显著的早熟和开花潜力的增强。在罗尔德植物中，早花之后伴随着大量侧花序的快速生长。对一些形态和组织学参数的分析表明，在Rold植株中观察到的一些特征性的形态异常可以用它们的早期生殖阶段转变来解释，并表明与未转化烟草相比，参与了植物体内更大部分的转变。此外，还对Rold植物组织的体外形态发生潜能进行了测试。在开花和无激素培养条件下，Rold植株的浅层细胞外植体表现出比对照更早和更强的花器官发生。
The Agrobacterium rhizogenes T-DNA oncogene rolD under the control of its own 5' regulatory region was transferred to day-neutral tobacco plants. The main trait induced by rolD in transgenic plants is a striking precocity in flower setting and a strong enhancement of the flowering potential. In rolD plants, early flowering is followed by the very rapid growth of numerous lateral inflorescences. The analysis of several morphological and histological parameters suggests that some characteristic morphological abnormalities observed in rolD plants can be accounted for by their early reproductive phase transition and points to the involvement in the transition of a greater portion of the plant body than is the case for untransformed tobacco. The in vitro morphogenic potential of tissues from rolD plants was also tested. Superficial thin cell layer explants from rolD plants show an earlier and much enhanced flower organogenesis, compared to controls, both on flowering and on hormone-free medium.