Assessing the limit of detection of Fourier-transform infrared spectroscopy and immunoassay strips for fentanyl in a real-world setting
Assessing the limit of detection of Fourier-transform infrared spectroscopy and immunoassay strips for fentanyl in a real-world setting
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DOI：
10.1111/dar.13004
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发表时间：
2019-11-19
影响因子：
3.8
通讯作者：
Ti, Lianping
中科院分区：
文献类型：
--
作者：
McCrae, Karen;Tobias, Samuel;Ti, Lianping
药物检查是一种减少危害的干预措施，越来越多地用于阿片类药物过量流行的背景下。本研究的目的是确定两种即时药物检查技术对芬太尼的检出限。设计和方法在护理点使用布鲁克傅里叶变换红外（FTIR）光谱和BTNX芬太尼免疫测定条进行测试的样品送至定量核磁共振（qNMR）光谱进行验证性实验室分析。测定了按重量计算的浓度，并与即时护理方法获得的结果进行了比较。结果共发送283份样品进行qNMR分析；其中173人（61.1%）芬太尼检测呈阳性。通过qNMR测定，芬太尼的重量浓度从1%到91%不等。173份样品中，FTIR未检出芬太尼的有30份（17.3%），试纸未检出芬太尼的有4份（2.3%）。FTIR检测不到的含有芬太尼的样品有浓度
Introduction and Aims Drug checking is a harm reduction intervention increasingly used in the context of the opioid overdose epidemic. The aim of the study was to determine the limit of detection for fentanyl of two point-of-care drug checking technologies. Design and Methods Samples tested at point-of-care using Bruker Fourier transform infrared (FTIR) spectroscopy and BTNX fentanyl immunoassay strips were sent for confirmatory laboratory analysis using quantitative nuclear magnetic resonance (qNMR) spectroscopy. Concentrations by weight were determined and compared to results obtained with point-of-care methods. Results In total, 283 samples were sent for qNMR analysis; among these, 173 (61.1%) tested positive for fentanyl. As determined by qNMR, fentanyl concentration by weight ranged from 1% to 91%. Among these 173 samples, fentanyl was not detected in 30 (17.3%) samples by FTIR and in 4 (2.3%) samples by test strip. Samples containing fentanyl that went undetected by FTIR had concentrations