Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old
Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old
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DOI：
10.1016/j.scr.2021.102607
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发表时间：
2021
影响因子：
1.2
通讯作者：
Sanbing Shen
中科院分区：
文献类型：
--
作者：
Min Liu;Ning Ge;Yongfeng Han;Jamie Reilly;Meimei Yang;Fan Yang;Janusz Krawczyk;Veronica McInerney;Timothy O’Brien;Terence Prendiville;Sanbing Shen
Skin punch biopsy was donated by a healthy 51-year-old Caucasian male and the dermal fibroblasts were reprogrammed into human induced pluripotent stem cell (hiPSC) lines by using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4 and c-MYC. Three iPSC lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) highly expressed the pluripotent markers and were capable of differentiating into cells of endodermal, mesodermal, and ectodermal origin. These iPSCs can be offered as controls and in combination with genome-editing and three-dimensional (3D) system. They may be used for human disease modelling and drug screening.
