A novel method for screening of potential probiotics for high adhesion capability
A novel method for screening of potential probiotics for high adhesion capability
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DOI：
10.3168/jds.2015-9356
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发表时间：
2015-07-01
影响因子：
3.5
通讯作者：
Chen, Tingtao
中科院分区：
文献类型：
--
作者：
Wang, Xin;Wu, Qinglong;Chen, Tingtao
为了筛选具有高粘附能力的潜在益生菌，采用基于恒化器模型的培养人粪便和变性梯度凝胶电泳方法，并在体外和体内评估分离株的粘附能力。从恒化器壁上的粘液中分离出植物乳杆菌（HM218749）、罗伊氏乳杆菌（EU547310）和粪肠球菌（HM218543），因为这些微生物在37℃的厌氧环境中可以更好地生长，并且可以抵抗恶劣的条件（pH 1.5和0.30%胆汁盐）。植物乳杆菌、罗伊氏乳杆菌和粪肠球菌可以粘附HT-29细胞，并减少痢疾志贺氏菌2457、金黄色葡萄球菌Cowanl、坂崎肠杆菌45401和大肠杆菌44102对HT-29细胞的粘附。此外，动物实验表明，植物乳杆菌可以粘附在小鼠肠道内，增加乳酸杆菌的数量，减少肠球菌的数量。
To screen for potential probiotics with high adhesion capability, a chemostat model-based cultured human feces and denaturing gradient gel electrophoresis methods were applied, and the adhesion capability of the isolates was evaluated in vitro and in vivo. Lactobacillus plantarum (HM218749), Lactobacillus reuteri (EU547310), and Enterococcus faecalis (HM218543) were isolated from the slime on the chemostat wall, as these organisms could grow better at 37 degrees C in an anaerobic environment and could resist harsh conditions (pH 1.5 and 0.30% bile salt). Lactobacillus plantarum, L. reuteri, and E. faecalis could adhere to HT-29 cells and reduce the adhesion of Shigella dysenteriae 2457, Staphylococcus aureus Cowanl, Enterobacter sakazakii 45401, and Escherichia coli 44102 to HT-29 cells. Moreover, the animal experiment showed that L. plantarum could adhere to mice intestine, increasing the number of lactobacilli and decreasing the number of enterococci.