Involvement of Satellite Cell Activation via Nitric Oxide Signaling in Ectopic Orofacial Hypersensitivity
Involvement of Satellite Cell Activation via Nitric Oxide Signaling in Ectopic Orofacial Hypersensitivity
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DOI：
10.3390/ijms21041252
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发表时间：
2020-02
影响因子：
5.6
通讯作者：
Jun Lee;Kinuyo Ohara;M. Shinoda;Yoshinori Hayashi;Asako Kubo;Shiori Sugawara;Sayaka Asano;Kumi Soma;Kohei Kanno;Masatoshi Ando;R. Koyama;Yuki Kimura;Kousuke Sakanashi;T. Iinuma;K. Iwata
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文献类型：
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作者：
Jun Lee;Kinuyo Ohara;M. Shinoda;Yoshinori Hayashi;Asako Kubo;Shiori Sugawara;Sayaka Asano;Kumi Soma;Kohei Kanno;Masatoshi Ando;R. Koyama;Yuki Kimura;Kousuke Sakanashi;T. Iinuma;K. Iwata
大鼠下牙槽神经切断（IANX）后，机械缩头阈值（MHWT）显著降低。硝酸盐和亚硝酸盐的合成显着增加三叉神经节（TG）在6小时后IANX。神经元型一氧化氮合酶（nNOS）免疫反应（IR）细胞的相对数量显着高于假手术和N-丙基-L-精氨酸（NPLA）治疗的IANX大鼠在IANX大鼠。在NPLA给药后第3天，IANX大鼠的MHWT显著恢复。L-精氨酸注入TG后，MHWT在15 min内显著减少，并且被胶质细胞酸性蛋白（GFAP）-IR细胞包围的TG细胞的平均数量显著增加。IANX组nNOS-IR细胞被GFAP-IR细胞包围的相对数量显著增加。与此相反，NPLA注射到TG后，GFAP-IR细胞的相对数量在IANX大鼠中显著减少。氟柠檬酸给药到TG显着减少GFAP-IR细胞的数量，并防止在IANX大鼠MHWT减少。目前的研究结果表明，IANX后，卫星胶质细胞被激活通过一氧化氮（NO）信号从TG神经元。三叉神经内的扩散性卫星胶质细胞活化导致面部区域的机械超敏反应，与三叉神经损伤没有直接关系。
The mechanical head-withdrawal threshold (MHWT) was significantly reduced following inferior alveolar nerve transection (IANX) in rats. Nitrate and nitrite synthesis was dramatically increased in the trigeminal ganglion (TG) at 6 h after the IANX. The relative number of neuronal nitric oxide synthase (nNOS)-immunoreactive (IR) cells was significantly higher in IANX rats compared to sham-operated and N-propyl-L-arginine (NPLA)-treated IANX rats. On day 3 after NPLA administration, the MHWT recovered considerably in IANX rats. Following L-arginine injection into the TG, the MHWT was significantly reduced within 15 min, and the mean number of TG cells encircled by glial fibrillary acidic protein (GFAP)-IR cells was substantially higher. The relative number of nNOS-IR cells encircled by GFAP-IR cells was significantly increased in IANX rats. In contrast, after NPLA injection into the TG, the relative number of GFAP-IR cells was considerably reduced in IANX rats. Fluorocitrate administration into the TG significantly reduced the number of GFAP-IR cells and prevented the MHWT reduction in IANX rats. The present findings suggest that following IANX, satellite glial cells are activated via nitric oxide (NO) signaling from TG neurons. The spreading satellite glial cell activation within the TG results in mechanical hypersensitivity of face regions not directly associated with the trigeminal nerve injury.