Instant Live-Cell Super-Resolution Imaging of Cellular Structures by Nanoinjection of Fluorescent Probes
Instant Live-Cell Super-Resolution Imaging of Cellular Structures by Nanoinjection of Fluorescent Probes
复制标题
DOI：
10.1021/nl504660t
复制
发表时间：
2015-02-01
期刊：
影响因子：
10.8
通讯作者：
Sauer, Markus
中科院分区：
文献类型：
--
作者：
Hennig, Simon;van de Linde, Sebastian;Sauer, Markus
用标准荧光探针标记活细胞内的内部结构是一个具有挑战性的问题。在这里，我们介绍了一种新的细胞内染色方法，使我们能够仔细控制标记过程，并提供即时访问活细胞的内部结构。使用直径为的中空玻璃毛细管
Labeling internal structures within living cells with standard fluorescent probes is a challenging problem. Here, we introduce a novel intracellular staining method that enables us to carefully control the labeling process and provides instant access to the inner structures of living cells. Using a hollow glass capillary with a diameter of