MOBILE MINOS ELEMENTS FROM DROSOPHILA-HYDEI ENCODE A 2-EXON TRANSPOSASE WITH SIMILARITY TO THE PAIRED DNA-BINDING DOMAIN
MOBILE MINOS ELEMENTS FROM DROSOPHILA-HYDEI ENCODE A 2-EXON TRANSPOSASE WITH SIMILARITY TO THE PAIRED DNA-BINDING DOMAIN
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DOI：
10.1073/pnas.91.11.4746
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发表时间：
1994-05-24
影响因子：
11.1
通讯作者：
SAVAKIS, C
中科院分区：
文献类型：
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作者：
FRANZ, G;LOUKERIS, TG;SAVAKIS, C
从果蝇hydei中克隆了与tc1样Mines移动元件相关的元件并进行了测序。Southern blot和序列分析表明：(i)这些元件在果蝇种系中活跃转座，（ii）它们具有显著的序列和大小同质性，（iii）与Tc1一样，它们插入可能在此过程中复制的TA二核苷酸上。两个元素Minos-2和Minos-3的核苷酸序列彼此之间只有一个位置不同，包含两个非重叠的开放阅读框，由一个假定的60个核苷酸内含子分隔。假设的Minos转座酶的氨基末端部分与配对的dna结合域序列相似。通过P元件介导的转化将修饰的Minos元件引入果蝇生殖系，强制转录导致精确拼接的聚腺苷化RNA分子的产生。有人提出，Minos-2和/或Minos-3可能编码一个活性转座酶，该转座酶含有与配对的dna结合域有远亲关系的氨基末端dna结合域。
Elements related to the Tc1-like Mines mobile element have been cloned from Drosophila hydei and sequenced. Southern blot and sequence analyses show that (i) the elements are actively transposing in the Drosephila hgdei germ line, (ii) they are characterized by a striking degree of sequence and size homogeneity, and (iii) like Tc1, they insert at a TA dinucleotide that is probably duplicated during the process. The nucleotide sequences of two elements, Minos-2 and Minos-3, differ at only one position from each other and contain two nonoverlapping open reading frames that are separated by a putative 60-nucleotide intron. The amino-terminal part of the Minos putative transposase shows sequence similarity to the paired DNA-binding domain. Forced transcription of a modified Minos element that was introduced into the Drosophila melanogaster germ line by P element-mediated transformation resulted in the production of accurately spliced polyadenylylated RNA molecules. It is proposed that Minos-2 and/or Minos-3 may encode an active transposase containing an amino-terminal DNA-binding domain that is distantly related to the paired DNA-binding domain.