Monitoring of adsorption behaviors of bovine serum albumin onto a stainless steel surface by the quartz crystal microbalance based on admittance analysis
Monitoring of adsorption behaviors of bovine serum albumin onto a stainless steel surface by the quartz crystal microbalance based on admittance analysis
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DOI：
10.1080/09168451.2017.1281724
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发表时间：
2017-04-01
影响因子：
1.6
通讯作者：
Sakiyama, Takaharu
中科院分区：
文献类型：
--
作者：
Hagiwara, Tomoaki;Nattawut, Phosri;Sakiyama, Takaharu
利用基于导纳分析的石英晶体微天平研究了牛血清白蛋白(BSA)在不锈钢表面的吸附过程。由于与BSA溶液的接触，吸附质量变化m随时间增加，达到m的渐近值和耗散因子D需要相当长的时间(>；2 h)。D与m的关系表明，BSA浓度越高，吸附的BSA分子层越坚硬。用Langmuir吸附等温式可以很好地描述2 h后m与最终BSA浓度之间的关系。然而，m的时间进程明显偏离了Langmuir吸附模型。扩展的指数函数模型很好地描述了m的时间过程，尽管它是一个经验模型。
The adsorption process of bovine serum albumin (BSA) onto a stainless steel surface was investigated using the quartz crystal microbalance based on admittance analysis. The adhered mass change m increased with time as a result of contacting the BSA solution, and considerably long period (>2 h) was required for the attainment of the asymptotic values of m as well as dissipation factor D. The relation between D and m suggested that the layer of adsorbed BSA molecules became stiffer with increasing time at higher BSA concentration. The relation between m after 2 h and the final BSA concentration was described well by the Langmuir adsorption isotherm. However, the time course of m clearly deviated from the Langmuir adsorption model. The stretched exponential function model described the time course of m well although it was an empirical one.