In situ hybridization analysis of human papillomavirus DNA segregation patterns in lesions of the female genital tract.
In situ hybridization analysis of human papillomavirus DNA segregation patterns in lesions of the female genital tract.
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女性生殖道病变中人乳头瘤病毒 DNA 分离模式的原位杂交分析。
DOI：
10.1016/0090-8258(90)90184-m
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发表时间：
1990
影响因子：
4.7
通讯作者：
Richart,RM
中科院分区：
文献类型：
--
作者：
Nuovo,GJ;Friedman,D;Richart,RM
不同的组织学特征可用于将生殖道的人乳头瘤病毒（HPV）相关病变分为两组：尖锐湿疣和“低度”或1级宫颈上皮内瘤变（CIN 1）与“高度”或2级和3级上皮内瘤变。采用原位杂交分析，我们在350例宫颈、外阴和肛周病变活检中将HPV DNA类型与组织学特征相关联。HPV DNA在外阴及肛周尖锐湿疣中的检出率最高（39 46，85%），而在CIN 1中的检出率为72%（86 12 0）。CIN 2 ~ 3级和外阴上皮内瘤变（VIN）2 ~ 3级的阳性率分别为5 3%（4 0 76）和5 7%（12 2 1）。39例肛周及外阴尖锐湿疣中，除2例HPV阳性外，其余均为6 ~ 11型。尽管它们的组织学特征相似，但在86例可检测到HPV的CIN 1病例中，只有23例（27%）的HPV类型为6 11，而86例中有31例（36%）含有HPV 16相关DNA，86例中有32例（37%）含有HPV 31，-33或-35相关DNA。CIN 2 3和VIN 2 3病变中的大多数病毒DNA与HPV 16相关，而CIN 2 3和VIN 2 3病变中没有HPV 6 11相关的DNA。结论：虽然皮肤生殖道尖锐湿疣与低致癌潜力的HPV（6型和11型）高度相关，但这些HPV类型在CIN 1病变中的发生率不如致癌HPV（16、31、33和35）。此外，HPV 6 - 11似乎很少与CIN 2 - 3或VIN 2 - 3病变相关。
Various histologic features may be used to divide human papillomavirus (HPV)-related lesions of the genital tract into two groups: condylomata and “low-grade” or grade 1 cervical intraepithelial neoplasias (CIN 1) versus “high-grade” or grade 2 and 3 intraepithelial neoplasias. Using in situ hybridization analysis we correlated HPV DNA type with histologic features in 350 biopsies of lesions from the cervix, vulva, and perianal region. HPV DNA was most commonly found in vulvar and perianal condylomata (39 46, 85%), whereas the rate in CIN 1 lesions was 72%(86 120). The rates were 53%(40 76) and 57%(12 21) in CIN 2 3 and vulvar intraepithelial neoplasm (VIN) grades 2 and 3, respectively. The HPV type in all but 2 of the 39 perianal and vulvar condylomata which contained HPV was 6 11. Despite their similar histologic features, the HPV type in only 23 of 86 (27%) CIN 1 cases with detectable HPV was 6 11 compared to 31 of 86 (36%) which contained HPV 16-related DNA and 32 of 86 (37%) which contained HPV 31,− 33, or− 35-related DNA. The viral DNA in the majority of CIN 2 3 lesions and all of the VIN 2 3 lesions was HPV-16 related; no CIN 2 3 or VIN 2 3 lesion had HPV 6 11-related DNA. It is concluded that although cutaneous genital tract condylomata are highly associated with HPVs of low oncogenic potential (types 6 and 11), these HPV types are not as frequent as the oncogenic HPVs (16, 31, 33, and 35) in CIN 1 lesions. Further, HPV 6 11 appears to be very rarely associated with CIN 2 3 or VIN 2 3 lesions.