Chemical composition and bioactivity studies of the essential oils from two Thymus species from the Pakistani flora
Chemical composition and bioactivity studies of the essential oils from two Thymus species from the Pakistani flora
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DOI：
10.1016/j.lwt.2012.06.003
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发表时间：
2013-01-01
影响因子：
6
通讯作者：
Sarker, Satyajit D.
中科院分区：
文献类型：
--
作者：
Hussain, Abdullah I.;Anwar, Farooq;Sarker, Satyajit D.
本研究的目的是评价两种巴基斯坦植物丁香和丁香精油的抗菌、抗氧化、抗疟疾和抗增殖活性。采用GC-MS联用技术对马尾松和马尾松挥发油进行分析，分别鉴定出48个和35个成分。丁香油的主要成分为百里香酚(36.5%)、香芹酚(9.50%)、乙酸胸腺酯(7.30%)、β-石竹烯(5.76%)，而丁香酚(44.4%)、邻聚伞花烃(14.0%)、α-松油醇(6.47%)、α-松油烯(6.06%)、β-石竹烯(5.25%)为主要成分。在纸片扩散法和Resazurin微量滴定法中，马尾松精油的抗菌活性明显好于马兜铃虫。这两种精油对耐苯氧西林的大肠埃希菌也有活性，尽管浓度相对较高。在DPPH测定中，蛇舌兰挥发油的抗氧化活性强于马尾松挥发油。两种精油都表现出较差的抗血红素生物结晶活性。在四甲基偶氮唑盐比色法中，马兜铃挥发油对MCF-7、LNCaP和NIH-3T3细胞的IC50值分别为80.7、70.3和100.2 mU g/ml，明显强于马兜铃虫挥发油。(C)2012爱思唯尔有限公司。保留所有权利。
The aim of this study was to evaluate the antibacterial, antioxidant, antimalarial and antiproliferative activities of the essential oils of two Pakistani plants, Thymus linearis and Thymus serpyllum of the family Lamiaceae. The GC-MS analyses of the essential oils of T. linearis and T. serpyllum led to the identification of 48 and 35 components, respectively. The major constituents of T linearis oil were thymol (36.5%), carvacrol (9.50%), thymyl acetate (7.30%), and beta-caryophyllene (5.76%), whereas, carvacrol (44.4%), o-cymene (14.0%), alpha-terpineol (6.47%), alpha-pinene (6.06%) and beta-caryophyllene (5.25%) were the main components of T. serpyllum oil. In the disc diffusion and resazurin microtitre assays, the essential oil of T linearis showed better antibacterial activity than that of T serpyllum. Both essential oils were also active, although at relatively high concentrations, against amphicillin-resistant Escherichia coli. In the DPPH assay, the essential oils of T. serpyllum showed more potent antioxidant activity than T linearis essential oil. Both essential oils exhibited poor anti-haem biocrystallisation activity. In the MTT assay, the anti-proliferative activity of T. linearis essential oil against MCF-7, LNCaP and NIH-3T3 cell lines in terms of IC50 were 80.7, 70.3 and 100.2 mu g/ml, respectively, which were notably stronger than T. serpyllum essential oil. (C) 2012 Elsevier Ltd. All rights reserved.