Peritumoral tissue compression is predictive of exudate flux in a rat model of cerebral tumor: an MRI study in an embedded tumor.
Peritumoral tissue compression is predictive of exudate flux in a rat model of cerebral tumor: an MRI study in an embedded tumor.
复制标题
DOI：
10.1002/nbm.3418
复制
发表时间：
2015-11
影响因子：
2.9
通讯作者：
Brown SL
中科院分区：
文献类型：
--
作者：
Ewing JR;Nagaraja TN;Aryal MP;Keenan KA;Elmghirbi R;Bagher-Ebadian H;Panda S;Lu M;Mikkelsen T;Cabral G;Brown SL
在脑肿瘤的实验模型中证实了对瘤周组织中细胞外体积和肿瘤渗出液流量的MRI估计。瘤周细胞外体积预测肿瘤渗出液流量。18只RNU无胸腺大鼠脑内接种U251 MG肿瘤细胞，并在植入后约18天进行动态对比增强MRI（DCE-MRI）研究。使用模型选择范式以及Patlak和Logan图对DCE-MRI数据的新应用，分布体积（即，组织孔隙度），以及从肿瘤内缘通过外缘的肿瘤渗出液流。外边缘的分布容积约为内相邻区域的一半（p < 1×10−4）。外环的分布容积与来自内缘的肿瘤渗出液流量显著相关（R2 = 0.9）。因此，肿瘤周围细胞外体积预测肿瘤渗出液流量的速率。对这些数据的一种解释是灌注，即，血液向肿瘤的输送受到肿瘤边缘大部分正常组织的压缩的调节，并且肿瘤渗出液流量受到肿瘤灌注的限制。在18只植入U251脑肿瘤的无胸腺大鼠中，从内环ROI（肿瘤）到外环ROI（主要是正常组织）的渗出液流量与外环ROI的细胞外体积（VD）。其中16项研究在24小时内重复进行。16项重复研究用虚线连接。这种非常显著的协方差（R2 = 0.9）意味着，边缘中的VD的知识将产生对渗出液流量的非常精确的预测，而不管其他参数，例如TIFP和肿瘤孔隙度，其可能被认为是预测渗出液流速所必需的。
MRI estimates of extracellular volume and tumor exudate flux in peritumoral tissue are demonstrated in an experimental model of cerebral tumor. Peritumoral extracellular volume predicted the tumor exudate flux. Eighteen RNU athymic rats were inoculated intracerebrally with U251MG tumor cells and studied with dynamic contrast-enhanced MRI (DCE-MRI) approximately 18 days post-implantation. Using a model selection paradigm and a novel application of Patlak and Logan plots to DCE-MRI data, the distribution volume (i.e., tissue porosity) in the leaky rim of the tumor, and in the tissue external to the rim (the outer rim), was estimated, as was the tumor exudate flow from the inner rim of the tumor through the outer rim. Distribution volume in the outer rim was approximately half that of the inner adjacent region (p < 1×10−4). The distribution volume of the outer ring was significantly correlated (R2 = 0.9) with tumor exudate flow from the inner rim. Thus, peritumoral extracellular volume predicted the rate of tumor exudate flux. One explanation for these data is that perfusion, i.e., the delivery of blood to the tumor, was regulated by the compression of the mostly normal tissue of the tumor rim, and that the tumor exudate flow was limited by tumor perfusion. Exudate flux from an inner ring ROI (tumor) to an outer ring ROI (mostly normal tissue) versus the extracellular volume (VD) of the outer ring ROI in 18 athymic rats with an embedded U251 cerebral tumor. Sixteen of the studies were repeated in a 24 hour interval. The 16 repeated studies are connected with dashed lines. This very significant co-variance (R2 = 0.9) implies that a knowledge of VD in the rim of the will yield a remarkably precise prediction of exudate flow, regardless of other parameters such as TIFP and tumor porosity, which might otherwise be thought necessary for a prediction of the exudate flow rate.