Radioimmunoassay of human parathyroid hormone in serum.
Radioimmunoassay of human parathyroid hormone in serum.
复制标题
血清中人甲状旁腺激素的放射免疫测定。
DOI：
10.1172/jci106476
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
T. Littledike
中科院分区：
文献类型：
--
作者：
C. Arnaud;H. S. Tsao;T. Littledike
本文介绍了一种新的人甲状旁腺激素(PTH)放射免疫测定法，可测定94%正常人血清中的甲状旁腺激素。它是基于人甲状旁腺激素与(131)I标记的牛甲状旁腺素竞争结合到针对猪甲状旁腺素的抗血清上的能力。该抗血清可区分从甲状旁腺腺瘤中提取的人甲状旁腺激素和甲状旁腺功能亢进患者血清中的甲状旁腺激素。有证据表明，这是由于从腺瘤中提取的激素的免疫化学变化，而不是由于血清中存在的激素的免疫化学异质性。提供了支持该分析的有效性和特异性的生理数据。诱发高钙血症和低钙血症可引起正常人和Paget病患者血清免疫反应性甲状旁腺激素(IPTH)的适当反应。在正常人中，血清iPTH在下午晚些时候和晚上逐渐增加，这表明了一种昼夜分泌节律。在血钙值正常范围内，血钙与血清iPTH呈负相关；在原发性甲状旁腺功能亢进症患者中，这两个变量之间呈正相关。正常人和原发性甲状旁腺功能亢进症患者的血清iPTH值之间有明显的重叠，但对血钙和iPTH值的正式判别分析表明，这两组人是分开的，没有重叠。
A new radioimmunoassay for human parathyroid hormone (PTH) in serum, which can measure the hormone present in 94% of the normal sera tested, is described. It is based on the ability of human PTH to compete with (131)I-labeled bovine PTH for binding to an antiserum directed against porcine PTH. This antiserum distinguishes between human PTH extracted from parathyroid adenomata and that present in hyperparathyroid sera. Evidence is given to suggest that this is due to immunochemical changes in the hormone extracted from adenomata and not to immunochemical heterogeneity of the hormone present in serum. Physiologic data supporting the validity and specificity of the assay are presented. Induced episodes of hypercalcemia and hypocalcemia resulted in appropriate responses in serum immunoreactive PTH (IPTH) in normal subjects and in patients with Paget's disease of bone. In normals, there was a progressive increase in serum IPTH in the late afternoon and evening, suggesting a diurnal secretory rhythm. A negative correlation was found between the serum calcium and serum IPTH over the normal range of serum calcium values; a positive correlation was found between these variables in patients with primary hyperparathyroidism. There was apparent overlap between serum IPTH values in normal subjects and patients with primary hyperparathyroidism, but formal discriminate analysis of values for serum calcium and IPTH demonstrated separation of these two groups, without overlap.