Multi-microarray identifies lower AQP9 expression in adjuvant chemotherapy nonresponders with stage III colorectal cancer
Multi-microarray identifies lower AQP9 expression in adjuvant chemotherapy nonresponders with stage III colorectal cancer
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多微阵列鉴定 III 期结直肠癌辅助化疗无反应者中 AQP9 表达较低
DOI：
10.1016/j.canlet.2013.04.017
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发表时间：
2013-08-09
期刊：
影响因子：
9.7
通讯作者：
Wang, Jianping
中科院分区：
文献类型：
--
作者：
Dou, Ruoxu;Deng, Yanhong;Wang, Jianping
大约25%的III期结直肠癌患者不能从标准辅助化疗中获益。为了鉴定无应答者的生物标志物，用基因表达和microRNA阵列分析了19名无应答者和16名应答者的新鲜冷冻组织。59个基因和17个miRNAs的差异表达至少2倍。AQP 9、SATB 2和WIF 1在无应答者中同时表达较低，并受到差异表达的miRNA的调节。RT-PCR验证了AQP 9的差异表达（p = 0.035）。结论：AQP 9基因表达降低与辅助化疗无反应有关，可作为肿瘤的预测指标和治疗靶点。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Approximately 25% of stage III colorectal cancer patients do not benefit from standard adjuvant chemotherapies. To identify biomarkers for nonresponders, fresh-frozen tissues of 19 nonresponders and 16 responders were analyzed with gene expression and microRNA arrays. Fifty-nine genes and 17 miRNAs were differentially expressed by at least two folds. AQP9, SATB2, and WIF1 were simultaneously lower expressed in the nonresponders and modulated by the differentially expressed miRNAs. RT-PCR validated the differential expression of AQP9 (p = 0.035). In conclusion, lower AQP9 gene expression is related with non-response to adjuvant chemotherapy, showing potential as predictive marker and therapeutic target. (C) 2013 Elsevier Ireland Ltd. All rights reserved.