Expression of HLA-DR by anagen hair follicles in alopecia areata.
Expression of HLA-DR by anagen hair follicles in alopecia areata.
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DOI：
10.1111/1523-1747.ep12277414
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发表时间：
1985-12
期刊：
影响因子：
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通讯作者：
A. G. Messenger;Stanley S. Bleehen
中科院分区：
文献类型：
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作者：
A. G. Messenger;Stanley S. Bleehen
用免疫过氧化物酶法研究了HLA-DR在斑秃毛囊内的表达。对12例斑秃患者和6例正常对照者进行了头皮活检。冷冻切片用4种抗hla - dr单克隆抗体，Leu 2、Leu 3、Leu 4和T6抗体进行染色。DR在正常毛囊和大多数非病变性脱发皮肤的毛囊中表达局限于树突状细胞，树突状细胞在直直毛毛插入水平以下稀疏。在病变皮肤中检查的37个毛囊中，有25个在皮层前基质和推定皮层的上皮细胞上显示DR染色。其他上皮室、下球根基质、内根鞘和外根鞘中有6个卵泡呈DR染色。大多数毛囊上皮细胞呈DR+，可见T细胞浸润毛囊基质。毛囊上皮DR抗原的异常表达为斑秃的发生提供了免疫机制的直接证据。在先前对斑秃的研究中，我们发现病变毛囊的细胞损伤局限于皮质前基质和假定的皮层。上皮细胞DR表达在同一部位的相对限制表明，卵泡的这一区域在疾病过程中具有根本的重要性。
The expression of HLA-DR within hair follicles in alopecia areata was studied using an immunoperoxidase method. Scalp biopsies were taken from 12 patients with alopecia areata and from 6 normal control subjects. Frozen sections were stained with a panel of 4 anti-HLA-DR monoclonal antibodies, Leu 2, Leu 3, Leu 4, and T6 antibodies. The expression of DR in normal hair follicles and in most anagen follicles from nonlesional alopecia skin was confined to dendritic cells which were sparse below the level of the arrector pilorum insertion. Of the 37 anagen follicles examined in lesional skin, 25 displayed staining for DR on epithelial cells in the precortical matrix and presumptive cortex. Six follicles showed DR staining in other epithelial compartments, the lower bulb matrix, inner root sheath, and outer root sheath. Infiltration of the hair bulb matrix by T cells was seen in the majority of follicles where epithelial cells were DR+. The aberrant expression of DR antigens by hair follicle epithelium provides direct evidence that immune mechanisms are operating in the pathogenesis of alopecia areata. In a previous study of alopecia areata we found evidence of cell injury confined to the precortical matrix and presumptive cortex in lesional anagen follicles. The relative restriction of epithelial DR expression to the same site suggests that this region of the follicle is of fundamental importance in the disease process.