Synthesis of 15N-enriched pseudouridine derivatives.
Synthesis of 15N-enriched pseudouridine derivatives.
复制标题
15N 富集假尿苷衍生物的合成。
DOI：
10.1021/ol035496e
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Chow,ChristineS
中科院分区：
文献类型：
--
作者：
Desaulniers,Jean-Paul;Ksebati,Bashar;Chow,ChristineS
A procedure for the chemical synthesis of [3-15N]-labeled pseudouridine and a methylated derivative was developed. A suitably protected pseudouridine precursor was nitrated at N3 followed by treatment with15NH4Cl to afford the15N-labeled product in six steps with a 20% yield. This methodology will allow for the production of RNAs with [3-15N]pseudouridine and [3-15N-methyl]pseudouridine at specific locations.
