The Fluidity of Phosphocholine and Maltoside Micelles and the Effect of CHAPS
The Fluidity of Phosphocholine and Maltoside Micelles and the Effect of CHAPS
复制标题
磷酸胆碱和麦芽糖苷胶束的流动性及 CHAPS 的影响
DOI：
10.1016/j.bpj.2019.03.019
复制
发表时间：
2019
影响因子：
3.4
通讯作者：
Columbus, Linda
中科院分区：
文献类型：
--
作者：
Kieber, Marissa;Ono, Tomihiro;Oliver, Ryan C.;Nyenhuis, Sarah B.;Tieleman, D. Peter;Columbus, Linda
采用自旋探针掺杂的电子顺磁共振方法研究了膜蛋白结构测定常用的洗涤剂磷脂胆碱和麦芽糖胶束的动力学。具体来说，磷酸胆碱是核磁共振研究中经常使用的洗涤剂，麦芽糖苷经常用于x射线晶体学结构测定。除了结构和静电差异之外，本研究旨在确定局部链动力学（即流动性）是否存在差异。氮氧化物探针在长链洗涤剂中的旋转动力学比在短链洗涤剂中的受限制更大，麦芽糖胶束比磷脂胆碱胶束受限制更大。此外，胶束微粘度可以通过混合物进行调节，如3-[（3-胆酰胺丙基）二甲酰胺]-1-丙磺酸与正十二烷基磷胆碱、正十四烷基磷胆碱、正癸基β- d -麦芽糖苷或正十二烷基β- d -麦芽糖苷的混合物。这些结果表明，除了已经确定的结构差异外，这些洗涤剂中膜蛋白稳定性的观察差异可能是由于流动性。
The dynamics of phosphocholine and maltoside micelles, detergents frequently used for membrane protein structure determination, were investigated using electron paramagnetic resonance of spin probes doped into the micelles. Specifically, phosphocholines are frequently used detergents in NMR studies, and maltosides are frequently used in x-ray crystallography structure determination. Beyond the structural and electrostatic differences, this study aimed to determine whether there are differences in the local chain dynamics (i.e., fluidity). The nitroxide probe rotational dynamics in longer chain detergents is more restricted than in shorter chain detergents, and maltoside micelles are more restricted than phosphocholine micelles. Furthermore, the micelle microviscosity can be modulated with mixtures, as demonstrated with mixtures of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate with n-dodecylphosphocholine, n-tetradecylphosphocholine, n-decyl-β-D-maltoside, or n-dodecyl-β-D-maltoside. These results indicate that observed differences in membrane protein stability in these detergents could be due to fluidity in addition to the already determined structural differences.