Sequence analysis and expression patterns of opsin genes in the longtooth grouper Epinephelus bruneus
Sequence analysis and expression patterns of opsin genes in the longtooth grouper Epinephelus bruneus
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文氏石斑鱼视蛋白基因序列分析及表达模式
DOI：
10.1007/s12562-015-0936-x
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发表时间：
2016
影响因子：
1.9
通讯作者：
Taro Matsumoto and Yasunori Ishibashi
中科院分区：
文献类型：
--
作者：
Okamoto H.;Ishikawa T.;Nishiki I.;Iwasaki Y.;Kai W. and Fujiwara A.;岡本裕之;Taro Matsumoto and Yasunori Ishibashi
在这里，以评估个体发育变化的视觉灵敏度在少年longtooth grouperEpinealus bruneus，我们调查了视蛋白基因序列和表达在幼虫和少年。长齿石斑鱼有10个视蛋白基因：Rh 1、5个Rh 2基因（Rh 2A 1 -1、Rh 2A 1 -2、Rh 2A 2、g6738和g6740）、SWS 1、2个SWS 2基因（SWS 2A 1和SWS 2A 2）和LWS。迄今为止，在鲈形目鱼类中已报道了5 ~ 6个Rh 2视蛋白基因。通过RT-PCR检测了视蛋白基因的差异表达;结果表明，SWS 1到SWS 2的转换发生在早期发育过程中，并且在孵化后14和40天之间没有LWS表达。视蛋白基因表达的这些个体发生变化可能对应于不同生命阶段的不同需求。
Here, to evaluate ontogenic changes in visual sensitivity in juvenile longtooth grouperEpinephelus bruneus, we investigated opsin gene sequences and expression in larvae and juveniles. The longtooth grouper has 10 opsin genes:Rh1, fiveRh2genes (Rh2A1-1,Rh2A1-2,Rh2A2,g6738, andg6740),SWS1, twoSWS2genes (SWS2A1andSWS2A2), andLWS. To date, five or sixRh2opsin genes have been reported in perciform fishes. Differential opsin gene expression was examined by RT-PCR; the results suggested that switching fromSWS1toSWS2occurs during early development, and that there is an absence ofLWSexpression between 14 and 40 days post-hatch. These ontogenetic changes in opsin gene expression may correspond to different requirements at different life stages.