Molecular characterization and expression pattern of insulin-like growth factor binding protein-3 (IGFBP-3) in common carp, Cyprinus carpio
Molecular characterization and expression pattern of insulin-like growth factor binding protein-3 (IGFBP-3) in common carp, Cyprinus carpio
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鲤鱼胰岛素样生长因子结合蛋白-3 (IGFBP-3) 的分子特征和表达模式
DOI：
10.1007/s10695-012-9681-6
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发表时间：
2012-12-01
影响因子：
2.9
通讯作者：
Li, Wensheng
中科院分区：
文献类型：
--
作者：
Chen, Wenbo;Lin, Haoran;Li, Wensheng
利用RT-PCR技术从鲤鱼肝脏中克隆了胰岛素样生长因子结合蛋白3（IGFBP-3）的全长cDNA。IGFBP-3 cDNA序列长1，680 bp，具有882 bp的开放阅读框，编码293个氨基酸残基的预测多肽。推导的氨基酸序列包含一个25个氨基酸残基的推定信号肽，导致268个氨基酸的成熟蛋白。经北方杂交分析，肝组织中出现约1.9kb的单一条带。IGFBP-3 mRNA在脑的所有区域都有高水平表达。在外周组织中，在视网膜、红肌、肝脏、心脏、后肠、脾脏和睾丸中发现高水平的IGFBP-3 mRNA。在白色肌肉、肾脏、胸腺和卵巢中IGFBP-3 mRNA的表达水平相对较低，而在头肾、血液、皮肤、鳃、中肠和前肠中IGFBP-3 mRNA的表达水平要低得多。IGFBP-3 mRNA在囊胚期首次被检测到，且表达水平显著升高。在原肠胚期，其含量急剧下降，随后逐渐上升。在生殖周期中，卵巢中IGFBP-3 mRNA的丰度在复发期和成熟期之间显著降低，而精巢中IGFBP-3 mRNA的表达量变化不显著。IGFBP-3基因在鲤鱼体内的表达谱表明，IGFBP-3在鲤鱼的发育和繁殖过程中可能发挥重要的生理功能。
A full-length cDNA encoding the insulin-like growth factor binding protein-3 (IGFBP-3) was cloned from the liver of common carp (Cyprinus carpio) by RT-PCR. The IGFBP-3 cDNA sequence is 1,680 bp long and has an open reading frame of 882 bp encoding a predicted polypeptide of 293 amino acid residues. The deduced amino acid sequence contains a putative signal peptide of 25 amino acid residues resulting in a mature protein of 268 amino acids. A single band of approximate 1.9 kb was found in liver by Northern blot analysis. IGFBP-3 mRNA was observed in all regions of brain with high levels. In peripheral tissues, high levels of IGFBP-3 mRNA were found in retina, red muscle, liver, heart, posterior intestine, spleen, and testis. Relatively lower levels were found in white muscle, kidney, thymus gland, and ovary, while in head kidney, blood, skin, gill, middle intestine, and anterior intestine, the IGFBP-3 mRNA levels were much lower. IGFBP-3 mRNA was first detected in the blastula stage with significantly high level. The level sharply decreased in gastrula stage, and it became to increase in the following stages. During the reproductive cycle, the abundance of IGFBP-3 mRNA significantly decreased between the recrudescing stage and the matured stage in ovary, although in testis, IGFBP-3 mRNA expression level did not exhibit a significant change. The mRNA expression profiles in the present study imply that the IGFBP-3 may play important physiological functions in common carp development and reproduction.