Development of a genetic manipulation system for Haemophilus parasuis
Development of a genetic manipulation system for Haemophilus parasuis
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DOI：
10.1016/j.vetmic.2004.10.015
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发表时间：
2005-02-25
影响因子：
3.3
通讯作者：
Llagostera, M
中科院分区：
文献类型：
--
作者：
Bigas, A;Garrido, ME;Llagostera, M
副猪嗜血杆菌（Haemophilus parasuis）是巴氏杆菌科（Pasteurellaceae）的一种重要病原菌，是引起猪格拉瑟氏病（Glasser's disease）的主要病原。因为没有遗传操作系统可用于H。迄今为止，在体内研究其基因参与毒力的作用是不可行的。在这里，我们证明了H。副猪链球菌具有环AIMP（cAMP）依赖性天然转化系统，其能够摄取其中必须存在ACCGAACTC序列信号的DNA。通过对外源DNA的浓度、cAMP浓度和暴露时间等DNA转化参数的优化，获得了一株H.副猪流感病毒在编码胸苷酸合成酶的thy基因中有缺陷。已经建成。这项工作中提供的数据为参与这种动物病原体感染过程的基因的功能分析提供了可能性。(C)2004 Elsevier B.V.保留所有权利。
Haemophilus parasuis is a member of the family Pasteurellaceae and an important respiratory-tract pathogen of swine, which is the etiological agent of Glasser's disease. Because no genetic manipulation system is available for H. parasuis so far, in vivo studies about the role of its genes involved in virulence are unfeasible. Here we demonstrate that H. parasuis has a cyclic AIMP (cAMP)-dependent natural transformation system that enables the uptake of DNA in which the ACCGAACTC sequence signal must be present. After improving DNA transformation parameters, such as cAMP and DNA concentration and exposition time of the exogenous DNA, a knockout mutant of H. parasuis defective in the thy gene, encoding the thymidylate synthase enzyme. has been constructed. Data presented in this work open the possibility for the functional analysis of genes involved in the infectious process of this animal pathogen. (C) 2004 Elsevier B.V. All rights reserved.