A novel mammalian Wnt gene, WNT8B, shows brain-restricted expression in early development, with sharply delimited expression boundaries in the developing forebrain
A novel mammalian Wnt gene, WNT8B, shows brain-restricted expression in early development, with sharply delimited expression boundaries in the developing forebrain
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DOI：
10.1093/hmg/7.5.813
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发表时间：
1998-05-01
影响因子：
3.5
通讯作者：
Strachan, T
中科院分区：
文献类型：
--
作者：
Lako, M;Lindsay, S;Strachan, T
我们目前对哺乳动物前脑发育的了解是贫乏的。相对较少的相关解剖标志，但是，正在补充基因表达的研究，能够确定解剖结构的子集。我们以前描述了克隆，亚染色体定位和初步结构表征的人WNT8B基因，第一个哺乳动物WNT8B基因被报道。Wnt基因编码细胞间信号分子，在早期发育中发挥各种关键作用，包括在某些情况下，在大脑发育中发挥假定的作用。在本报告中，我们提出了全长cDNA序列和人类Wnt8b基因的基因组组织和报告的Wnt8b基因在人类和小鼠胚胎中的表达研究。人类和小鼠的表达模式似乎是相同的，并且仅限于发育中的大脑，其中绝大多数表达存在于发育中的前脑中。在后一种情况下，表达仅限于三个明确界定的区域的生发神经上皮：端脑脑室（其中包括发育中的海马）的背内侧壁，背侧丘脑和后下丘脑的乳头状和后乳头状区域的离散区域。在发育中的海马体中的表达可能表明WNT8B在该区域的模式化中的作用，并且人类基因在10q24的亚染色体定位可能表明其在疾病与该区域中的标记物相关联的家庭中作为部分癫痫的候选基因。
Our current knowledge of mammalian forebrain development is meagre. The comparatively few relevant anatomical landmarks are, however, being supplemented by gene expression studies which are able to identify subsets of anatomical structures. We previously described cloning, subchromosomal localization and preliminary structural characterization of the human WNT8B gene, the first mammalian Wnt8b gene to be reported. Wnt genes encode intercellular signalling molecules which play a variety of critical roles in early development, including, in several cases, a presumed role in brain development. In the current report we present the full-length cDNA sequence and genomic organization of the human Wnt8b gene and report studies of expression of the Wnt8b gene in human and mouse embryos. The human and mouse expression patterns appeared identical and were restricted to the developing brain, with the great majority of expression being found in the developing forebrain. In the latter case expression was confined to the germinative neuroepithelium of three sharply delimited regions: the dorsomedial wall of the telencephalic ventricles (which includes the developing hippocampus), a discrete region of the dorsal thalamus and the mammillary and retro-mammillary regions of the posterior hypothalamus. Expression in the developing hippocampus may suggest a role for WNT8B in patterning of this region and subchromosomal localization of the human gene to 10q24 may suggest it as a candidate gene for partial epilepsy in families where the disease has been linked to markers in this region.