DNA from Drosophila melanogaster beta-heterochromatin binds specifically to nuclear lamins in vitro and the nuclear envelope in situ
DNA from Drosophila melanogaster beta-heterochromatin binds specifically to nuclear lamins in vitro and the nuclear envelope in situ
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DOI：
10.1016/0378-1119(96)00002-9
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发表时间：
1996-06-01
期刊：
影响因子：
3.5
通讯作者：
Fisher, PA
中科院分区：
文献类型：
--
作者：
Baricheva, EA;Berrios, M;Fisher, PA
一个名为lambda 20p1.4的DNA片段在体外与聚合的果蝇Lamin结合。将lambda 20p1.4与分离的多线染色体进行原位杂交，发现定位于2号染色体右臂的染色质中心和49cd区域。每个单倍体基因组可检测到约120个拷贝的与lambda 20p1.4同源的序列。核苷酸(NT)序列分析表明，lambda 20p1.4是一个富含A+T的1327个碱基的片段，包含4个重复单位，在nt 595和919之间。结果表明，Lamin与lambda 20p1.4区域相互作用，该区域在300到1000个核苷酸之间。共聚焦免疫荧光共定位结果显示，在原位杂交中，lambda 20p1.4的主要位置，即染色中心，与核膜(板层)并列。这是首次证明，在体外与核层特异结合的DNA序列位于核膜的原位或附近，并可能位于体内。
A DNA fragment designated lambda 20p1.4 binds in vitro to polymerized Drosophila melanogaster lamin. In situ hybridization of lambda 20p1.4 to isolated polytene chromosomes revealed localization at the chromocenter and to the 49 CD region on the right arm of chromosome 2. About 120 copies of sequences homologous to lambda 20p1.4 were detected per haploid genome. Nucleotide (nt) sequence analysis demonstrates that lambda 20p1.4 is an A+T-rich, 1327-bp fragment containing four repeated units between nt 595 and 919. Results suggest that lamin interacts with a region of lambda 20p1.4 between nt 300 and 1000. Confocal immunofluorescence co-localization demonstrates that in situ, the major locus of lambda 20p1.4 hybridization, the chromocenter, is found juxtaposed to the nuclear envelope (lamina). This is the first demonstration that a DNA sequence that binds specifically to nuclear lamins in vitro, is located at or near the nuclear envelope in situ and, presumably, in vivo.