The Nup153-Nup50 Protein Interface and Its Role in Nuclear Import
The Nup153-Nup50 Protein Interface and Its Role in Nuclear Import
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DOI：
10.1074/jbc.m112.378893
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发表时间：
2012-11-09
影响因子：
4.8
通讯作者：
Ullman, Katharine S.
中科院分区：
文献类型：
--
作者：
Makise, Masaki;Mackay, Douglas R.;Ullman, Katharine S.
Nup50和可溶性转运因子之间的相互作用是某些核细胞质转运途径效率的基础。这些相互作用发生的平台对于建立对核细胞质贩运的全面了解非常重要。Nup153是为Nup50提供支架的核孔蛋白。在这里，我们描述了Nup153和Nup50之间相互作用的需求，揭示了一个双重界面。Nup50与Nup153独特的n端区域的相互作用对于Nup50的核孔定位至关重要。第二个相互作用位点位于Nup153的远端尾部，依赖于α的输入。这两种相互作用都涉及Nup50的n端结构域。Nup153-Nup50伙伴关系的结构表明，Nup153支架不仅为Nup50提供了一种孔隙靶向手段，而且还提供了一个局部环境，促进Nup50和输入α蛋白在一起，以及参与运输的其他可溶性因子。与此一致的是，Nup153-Nup50界面的破坏降低了核进口的效率。
Interactions between Nup50 and soluble transport factors underlie the efficiency of certain nucleocytoplasmic transport pathways. The platform on which these interactions take place is important to building a complete understanding of nucleocytoplasmic trafficking. Nup153 is the nucleoporin that provides this scaffold for Nup50. Here, we have delineated requirements for the interaction between Nup153 and Nup50, revealing a dual interface. An interaction between Nup50 and a region in the unique N-terminal region of Nup153 is critical for the nuclear pore localization of Nup50. A second site of interaction is at the distal tail of Nup153 and is dependent on importin alpha. Both of these interactions involve the N-terminal domain of Nup50. The configuration of the Nup153-Nup50 partnership suggests that the Nup153 scaffold provides not just a means of pore targeting for Nup50 but also serves to provide a local environment that facilitates bringing Nup50 and importin alpha together, as well as other soluble factors involved in transport. Consistent with this, disruption of the Nup153-Nup50 interface decreases efficiency of nuclear import.