The CD39-like gene family:: Identification of three new human members (CD39L2, CD39L3, and CD39L4), their murine homologues, and a member of the gene family from Drosophila melanogaster
The CD39-like gene family:: Identification of three new human members (CD39L2, CD39L3, and CD39L4), their murine homologues, and a member of the gene family from Drosophila melanogaster
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DOI：
10.1006/geno.1998.5317
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发表时间：
1998-06-15
期刊：
影响因子：
4.4
通讯作者：
Frischauf, AM
中科院分区：
文献类型：
--
作者：
Chadwick, BP;Frischauf, AM
人类淋巴细胞激活抗原CD39是一种已知的E型apyrase，它能降解细胞外的ATP和ADP，在同型黏附、血小板聚集和激活的淋巴细胞清除ATP的溶解作用中起着重要的作用。最近发现的CD39L1的大鼠同源物，通过水解胞外ATP，被证明具有E-型胞外-ATPase活性。我们已经鉴定了三个新的CD39样转录本，CD39L2，CD39L3和CD39L4，它们与脊椎动物、无脊椎动物和植物中的其他核苷酸三磷酸酶具有广泛的氨基酸同源性，表明这些基因也编码具有胞外核苷酸酶活性的蛋白质。每个基因的全长cDNA克隆的分离和测序确定了485、529和429个氨基酸的推测蛋白质。分析了该基因家族中所有5个人类成员的表达模式。将CD39L2、CD39L3和CD39L4定位在人类基因组上，并根据人类和小鼠染色体之间保守的基因序列区域，对定位有可能的小鼠同源基因进行了归属。Mcd3914的MAP定位将该基因定位于与小鼠听源性惊厥易感性相关的区域。这种疾病的特征是由响亮的高频声音引起的惊厥，并已被证明与核苷酸三磷酸酶活性增加有关。(C)1998年学术出版社。
The human lymphoid cell activation antigen CD39 is a known E-type apyrase that hydrolyzes extracellular ATP and ADP, a function important in homotypic adhesion, platelet aggregation, and removal by activated lymphocytes of the lytic effect of ATP. The recently identified putative rat homologue of CD39L1 has been shown to have E-type ecto-ATPase activity, by hydrolyzing extracellular ATP. We have characterized three novel CD39-like transcripts, CD39L2, CD39L3, and CD39L4, which share extensive amino acid homology with other nucleotide triphosphatases in vertebrates, invertebrates, and plants, suggesting that these genes also encode proteins with ecto-nucleotidase activity. Isolation and sequencing of full-length cDNA clones for each gene identified putative proteins of 485, 529, and 429 amino acids. The expression pattern of all five human members of the gene family was analyzed. CD39L2, CD39L3, and CD39L4 were mapped on the human genome, and the murine homologues identified with the putative map locations were assigned on the basis of regions of conserved gene order between human and mouse chromosomes. The map location of mcd3914 places the gene within a region associated with audiogenic seizure susceptibility in mouse. This disorder is characterized by convulsions induced by loud high-frequency sound and has been shown to be associated with increased nucleotide triphosphatase activity. (C) 1998 Academic Press.