THE DIFFERENTIATION OF OLIGODENDROCYTES IN A SERUM-FREE HORMONE-SUPPLEMENTED MEDIUM
THE DIFFERENTIATION OF OLIGODENDROCYTES IN A SERUM-FREE HORMONE-SUPPLEMENTED MEDIUM
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DOI：
10.1016/0165-3806(84)90056-7
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
SILBERBERG, DH
中科院分区：
文献类型：
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作者：
ECCLESTON, PA;SILBERBERG, DH
胰蛋白酶分离的胎儿和新生大鼠脑和脊髓的原代混合培养物在无血清培养基中生长。该培养基含有胰岛素、硒、转铁蛋白和三碘甲状腺原氨酸，通过测定表达表面半乳脑苷脂的细胞数量，针对少突胶质细胞的存活进行了优化。含血清和无血清培养基中培养物的比较表明，在没有血清的情况下，可以更早地检测到半乳糖脑苷脂阳性（GalC+）少突胶质细胞。这种早期分化是在没有添加激素和营养物质的情况下发生的，其主要功能是延长细胞的存活时间。通过比较含有2.5%和10%胎牛血清的培养基中表面GalC的表达，研究血清对少突胶质细胞分化的影响。在给定时间，在较低血清浓度下检测到更多数量的 GalC+ 少突胶质细胞。当培养物从10%血清转移到无血清培养基（或1%血清）时，随后出现大量GalC+少突胶质细胞，表明前体细胞存在于高血清培养基中，但它们无法分化。讨论了血清对少突胶质细胞分化影响的可能解释。
Primary mixed cultures of trypsin-dissociated fetal and newborn rat brain and spinal cord were grown in a serum-free medium. This medium, containing insulin, selenium, transferrin and triiodothyronine, was optimized for oligodendrocyte survival by determining the number of cells which expressed surface galactocerebroside. Comparison of cultures in serum-containing and serum-free media revealed that galactocerebroside positive (GalC+) oligodendrocytes could be detected earlier in the absence of serum. This early differentiation occurred in the absence of the added hormones and nutrients, whose main function was to prolong survival of the cells. The effect of serum on the differentiation of oligodendrocytes was studied by comparing the expression of surface GalC in media containing 2.5% or 10% fetal calf serum. At a given time a much greater number of GalC+ oligodendrocytes was detected at the lower serum concentration. When cultures were transferred from 10% serum to serum-free medium (or 1% serum) large numbers of GalC+ oligodendrocytes subsequently appeared, showing that precursors were present in the high-serum medium, but that they were unable to differentiate. Possible explanations of the effect of serum on oligodendrocyte differentiation are discussed.