Molecular cloning and sequencing of a cDNA of rat dopa decarboxylase: partial amino acid homologies with other enzymes synthesizing catecholamines.
Molecular cloning and sequencing of a cDNA of rat dopa decarboxylase: partial amino acid homologies with other enzymes synthesizing catecholamines.
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大鼠多巴脱羧酶 cDNA 的分子克隆和测序：与其他合成儿茶酚胺的酶具有部分氨基酸同源性。
DOI：
10.1073/pnas.86.20.8142
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发表时间：
1989
影响因子：
11.1
通讯作者：
H. Wada
中科院分区：
文献类型：
--
作者：
T. Tanaka;Y. Horio;M. Taketoshi;I. Imamura;M. Ando;K. Kangawa;H. Matsuo;M. Kuroda;H. Wada
从大鼠肝脏中纯化多巴脱羧酶（DDC; aromatic-L-amino-acid decarboxylase; aromatic-L-amino-acid carboxylase，EC 4.1.1.28），并测定其部分序列。用人工合成的寡核苷酸构建大鼠肝脏cDNA文库，筛选出3个克隆并进行测序。所克隆的2个DDCcDNA片段均由78个核苷酸的5 '端非编码区、1440个核苷酸的编码区和438个核苷酸的3'端非编码区组成。编码的蛋白质由480个氨基酸残基组成，分子量为54，000。大鼠DDC一级结构的一个特殊特征是由29个氨基酸残基组成的重复结构。一个58个氨基酸残基的序列，包括大鼠DDC的重复结构，被发现与大鼠酪氨酸羟化酶，人多巴胺β-羟化酶，牛苯乙醇胺N-甲基转移酶，其他哺乳动物的酶，合成儿茶酚胺的同源性。这些结果表明，儿茶酚胺生物合成酶的结构相关，并建议其同源结构域是重要的儿茶酚-蛋白质相互作用。
Dopa decarboxylase (DDC; aromatic-L-amino-acid decarboxylase; aromatic-L-amino-acid carboxylase, EC 4.1.1.28) was purified from rat liver and its partial sequence was determined. Synthetic oligonucleotides were used to construct and screen rat liver cDNA libraries, and three clones were isolated and sequenced. The 2 kilobases of DDC cDNA cloned consisted of a 5'-noncoding segment of 78 nucleotides, a coding region of 1440 nucleotides, and a 3'-noncoding region of 438 nucleotides. The encoded protein of 480 amino acid residues had a molecular weight of 54,000. A special feature of the primary structure of rat DDC was a repeating structure consisting of 29 amino acid residues. A sequence of 58 amino acid residues, including this repeating structure of rat DDC, was found to show homologies with those of rat tyrosine hydroxylase, human dopamine beta-hydroxylase, and bovine phenylethanolamine N-methyltransferase, other mammalian enzymes that synthesize catecholamines. These results indicate that catecholamine biosynthetic enzymes are structurally related and suggest that their homologous domains are important for catechol-protein interactions.
儿茶酚胺生物合成酶同源基因编码区存在的证据。
DOI：
10.1101/sqb.1983.048.01.036
发表时间：
1983
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Joh,TH;Baetge,EE;Ross,ME;Reis,DJ
通讯作者：
Reis,DJ