VISUAL network analysis reveals the role of BEH3 as a stabilizer in the secondary vascular development in Arabidopsis
VISUAL network analysis reveals the role of BEH3 as a stabilizer in the secondary vascular development in Arabidopsis
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VISUAL网络分析揭示BEH3作为稳定剂在拟南芥次生血管发育中的作用
DOI：
10.1101/2021.01.19.427273
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Kondo Yuki
中科院分区：
文献类型：
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作者：
Furuya Tomoyuki;Saito Masato;Uchimura Haruka;Satake Akiko;Nosaki Shohei;Miyakawa Takuya;Shimadzu Shunji;Yamori Wataru;Tanokura Masaru;Fukuda Hiroo;Kondo Yuki
在植物次生生长过程中，位于形成层的维管干细胞在整个生命周期中不断进行自我更新和分化。最近的细胞分选技术能够揭示形成层细胞的转录调控框架。然而，血管干细胞的强大控制机制尚不清楚。通过使用拟南芥子叶的异位维管细胞转分化系统的多个转录组数据集进行共表达网络分析，我们从另一种方法中发现了一个形成层特异性基因模块。形成层基因列表包括转录因子BES1/BZR1同源物3 (BEH3)，其同源物BES1已知负向控制血管干细胞维持。有趣的是，BEH3突变体的血管大小表现出很大的变化，这意味着BEH3作为稳定剂的作用。BEH3几乎失去了转录抑制活性，并通过与相同基序BRRE的竞争性结合与其他BES/BZR成员拮抗。事实上，数学模型表明，BES/BZRs之间的竞争关系导致了血管干细胞的强大调节。
During secondary growth in plants, vascular stem cells located in the cambium continuously undergo self-renewal and differentiation throughout the lifetime. Recent cell-sorting technique enables to uncover transcriptional regulatory framework for cambial cells. However, the mechanisms underlying the robust control of vascular stem cells have not been understood yet. By coexpression network analysis using multiple transcriptome datasets of an ectopic vascular cell transdifferentiation system using Arabidopsis cotyledons, VISUAL, we newly identified a cambium-specific gene module from an alternative approach. The cambium gene list included a transcription factor BES1/BZR1 homolog 3 (BEH3), whose homolog BES1 is known to control vascular stem cell maintenance negatively. Interestingly, the vascular size of thebeh3mutants showed a large variation, implying the role of BEH3 as a stabilizer. BEH3 almost lost the transcriptional repressor activity and functioned antagonistically with other BES/BZR members via competitive binding to the same motif BRRE. Indeed, mathematical modeling suggests that the competitive relationship among BES/BZRs leads to the robust regulation of vascular stem cells.