Quantifying blood leakage into the oral mucosa and its effects on the measurement of cortisol, dehydroepiandrosterone, and testosterone in saliva
Quantifying blood leakage into the oral mucosa and its effects on the measurement of cortisol, dehydroepiandrosterone, and testosterone in saliva
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DOI：
10.1016/j.yhbeh.2004.01.006
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发表时间：
2004-06-01
影响因子：
3.5
通讯作者：
Shirtcliff, EA
中科院分区：
文献类型：
--
作者：
Kivlighan, KT;Granger, DA;Shirtcliff, EA
研究口腔微损伤引起的漏血对唾液激素测定的影响。在强力刷牙之前、之后以及每15分钟1小时采集一次唾液样本。唾液中的血液通过目测染色、Hemastix(R)试剂条检测血红蛋白和免疫法检测转铁蛋白来定量。所有方法均证实微损伤后即刻唾液中有血。血红蛋白和转铁蛋白水平高于基线至少30分钟。微损伤后，唾液睾酮水平高于基线水平，并持续升高30分钟。微损伤引起的唾液睾酮变化与转铁蛋白的变化的关系比血红蛋白水平或变色等级的关系更密切。平均而言，唾液脱氢表雄酮（DHEA）水平对微损伤的反应没有增加。然而，微损伤引起的脱氢表雄酮变化的个体差异与变色等级有关。平均而言，唾液皮质醇水平对微损伤没有反应，皮质醇变化的个体差异也与血液污染措施无关。基线激素水平的昼夜差异和性别差异都不能预测漏血对定量唾液测量的影响。研究结果表明，生态学上有效的口腔轻度至中度微损伤对唾液皮质醇测量的影响可以忽略不计，但在评估其他激素（尤其是睾酮）时，可能对量化和控制很重要。(C) 2004爱思唯尔公司版权所有。
The impact of blood leakage due to microinjury to the oral cavity on the measurement of salivary hormones was examined. Saliva samples were collected before, immediately after, and then every 15 min for 1 h following vigorous tooth brushing. Blood in saliva was quantified by visual inspection of discoloration, Hemastix(R) reagent strips to detect hemoglobin, and an immunoassay for transferrin. The presence of blood in saliva immediately after microinjury was confirmed by all methods. Hemoglobin and transferrin levels remained elevated over baseline for at least 30 min. Levels of salivary testosterone increased over baseline and remained elevated for 30 min in response to microinjury. Microinjury induced change in salivary testosterone was more closely associated with the change in transferrin than hemoglobin levels or discoloration ratings. On average, levels of salivary dehydroepiandrosterone (DHEA) did not increase in response to microinjury. However, individual differences in microinjury induced change in DHEA were associated with discoloration ratings. Salivary cortisol levels, on average, were neither responsive to microinjury, nor were individual differences in cortisol change associated with blood contamination measures. Neither diurnal nor gender-related differences in baseline hormone levels predicted the impact of blood leakage on quantitative salivary measurements. The findings suggest ecologically valid minor-to-moderate level microinjuries to the oral cavity have negligible effects on the measurement of salivary cortisol, but may be important to quantify and control when assessing other hormones especially testosterone. (C) 2004 Elsevier Inc. All rights reserved.